Method for Measuring Gap Junctional Intercellular Communication by Fluorescence Recovery after Photobleaching.

• Luo Shenqiu

  Department of Cell Biology and Medical Genetics, First Military Medical University

• Mo Yongyan

  Department of Cell Biology and Medical Genetics, First Military Medical University

• Seguchi Harumichi

  Department of Anatomy and Cell Biology, Kochi Medical School

抄録

The present study was designed to establish a method for measuring cultured neuroglial gap junctional intercellular communication (GJIC) of rat cerebral cortices by fluorescence recovery after photobleaching (FRAP) analysis technique. As a result of the managements, such as cell culture, fluorescence staining, fluorescence excitement, laser scanning and computer analysis et al., fluorescence inside all selected cells recovered in different degrees, i. e., fluorescence relative intensities in the 1st-5th cell raised 14%, 28%, 43%, 17.5% and 12.5% respectively after scanning 15.4 min, the mean value of recovery was 18.57±10.06% and the mean rate of fluorescence recovery was 1.223±0.785%/min for three samples (n=14 cells). The result showed that the FRAP is an ideal method for measuring GJIC.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 30 (5/6), 575-578, 1997

  日本組織細胞化学会

キーワード

• FRAP
• Communication
• Gap junction

詳細情報

• CRID: 1390001204863338624
• NII論文ID: 10008607952
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.30.575
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/30/5-6/30_5-6_575/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1998181470
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可