Immunolocalization of Aldolase A Subunit Using Monoclonal Antibody in Rabbit Tissues

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We prepared a monoclonal antibody (mAb), IgM class, to rabbit muscle aldolase (ALD) A<SUB>4</SUB>. The mAb reacted with the ALD A subunit but not with the B and C subunits. Distribution of the ALD A subunit was studied employing immunohistochemistry, with the ABC procedure in the rabbit tissues. The ALD A subunit was localized in the l-band of the skeletal muscle fibers. In the liver, endothelial cells showed strong immunoreactivity while parenchymal cells were negative or yielded only a faint reaction. The collecting tubules were positively stained in the kidney. Strong reactivity was seen in the proximal tubules of the outer medulla but not in those of the cortex. In the adrenal gland, cells in the cortex exhibited strong immunostaining, while medullary cells were negative. In the testis, most of the seminiferous epithelium was moderately immunoreactive. Intense staining was present in interstitial cells. The ALD A subunit was localized in the interstitial cells and follicular cells of primary follicles in the ovary. In the cerebellum, the ALD A subunit was localized exclusively in nerve fibers of the white matter; Purkinje cells and nerve cells in the granular layer were negative.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 30(5), 601-608, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

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各種コード

  • NII論文ID(NAID)
    10008608002
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  J-STAGE 
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