無細胞半合成培地を用いる鼠癩菌分離培養の試み

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  • Attempts for Cultivation of <i>Mycobacterium lepracmurium</i> in Cell-Free, Semi-Synthetic Soft Agar Medium
  • ムサイボウ ハンゴウセイバイチ オ モチイル ネズミライキン ブンリ バイヨウ ノ ココロミ

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A biological significance was suggested to be shown by an acid-fastness map devised from investigation of the effect of ultraviolet irradiation on the acid-fastness of mycobacteria. Then, attempts were made to cultivate Mycobacterium lepraemurium Hawaii from nodules of infected mice in a cell-free, semi-synthetic soft agar medium. The basic composition of this medium was modified to some extent from that used for the successul cultivatioh of M. paratuberculosis Johnei.<br>Experiments were carried out repeatedly five times. Those organisms which had multiplied even to a limited extent were observed to form microcolony-like masses, which were composed of elongated, strongly acid-fast rods. It was interesting to note that when a medium contained both glycerol and glucose as carbon sources in addition to ferricion, it seemed to support the growth of M. lepraemurium to some extent.

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