Kinetics for Camel (Camelus dromedarius) Retina Acetylcholinesterase Inhibition by Methotrexate In Vitro

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This work addresses the kinetic analysis of the interaction of methotrexate (MTX) with camel retina acetylcholinesterase (AChE, EC 3. 1. 1. 7). It was found that the MTX effect was reversible in nature. The IC<SUB>50</SUB> was determined, by two methods, to be 1.362 mM. The Michaelis-Menten constant (K<SUB>s</SUB>) for the hydrolysis of acetylthiocholine iodide (ASCh) by AChE was 0.123 mM in the control system, and the MTX-treated systems showed a 10-35% decrease in this value. The V<SUB>max</SUB> was 0.789 μmol/min/mg protein for the control system, while it was decreased by 23-76% in the MTX-treated systems. The Lineweaver-Burk plot, Dixon plot and their secondary replots indicated that the inhibition was a linear mixed type; i.e., uncompetitive and noncompetitive. The values of K<SUB>i</SUB> and K<SUB>I</SUB> were estimated as 0.782 and 0.404 mM, respectively. The use of camel retina as a model for the study of human retina may open new avenues for studying various aspects of AChE.

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  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 72(1), 49-55, 1996-09-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008675637
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • データ提供元
    CJP書誌  J-STAGE 
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