Ouabain : Induced Cell Proliferation in Cultured Rat Astrocytes

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Ouabain markedly stimulated not only [<SUP>3</SUP>H]thymidine incorporation but also [<SUP>3</SUP>H]uridine incorporation into astrocytes. The effects were observed at 36-48 hr and 12-72 hr after addition of ouabain, respectively. The dose-response curves were both bell-shaped types with a peak at 10<SUP>−3</SUP> M for thymidine incorporation and 2 × 10<SUP>−3</SUP> M for uridine incorporation. Ouabain increased cell number as determined by an assay using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide and by a method using a hemocyiometer. Low concentration of external K<SUP>+</SUP> mimicked the effect of ouabain in stimulating [<SUP>3</SUP>H]-thymidine incorporation, and high concentration of external K<SUP>+</SUP> blocked the effect of ouabain. In contrast to astrocyies, ouabain did not stimulate [<SUP>3</SUP>H]thymidine incorporation into C6 glioma and fibroblast cells. The effect of ouabain on [<SUP>3</SUP>H]thymidine incorporation in astrocyies was dependent on external Ca<SUP>2+</SUP>, and it was blocked by cycloheximide. These findings indicate that prolonged Na<SUP>+</SUP>, K<SUP>+</SUP>-ATPase inhibition causes cell proliferation in cultured astrocyies in cell-specific and Ca<SUP>2+</SUP>-dependent manners.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 72(4), 347-353, 1996-12-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008676581
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4121853
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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