Oxidative Stress-Induced Increase in Intracellular Ca2+ and Ca2+-Induced Increase in Oxidative Stress: An Experimental Model Using Dissociated Rat Brain Neurons.

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  • Oyama Yasuo
    <I>Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima</I>
  • Okazaki Eisuke
    <I>Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima</I>
  • Chikahisa Lumi
    <I>Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima</I> <I>Cancer Research Laboratory, Taiho Pharmaceutical Co., Ltd.</I>
  • Nagano Takayuki
    <I>Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima</I>
  • Sadakata Chiharu
    <I>Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima</I>

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タイトル別名
  • Oxidative Stress Induced Increase in In

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In order to study the oxidative stress-induced change in intracellular concentration of Ca2+ ([Ca2+]i) and Ca2+-induced oxidative stress, effects of hydrogen peroxide and ionomycin, a calcium ionophore, on rat cerebellar neurons were examined using a flow cytometer and fluorescent dyes: fluo-3 for monitoring [Ca2+]i; 2'', 7''-dichlorofluorescin, for reactive oxygen species; and 5-chloromethylfiuorescein, for cellular nonprotein thiols. Oxidative stress induced by hydrogen peroxide dose-dependently increased [Ca2+]i and decreased the content of nonprotein thiols. Ionomycin increased oxidative metabolism and decreased the content of nonprotein thiols. Results suggest that oxidative stress induces an increase in [Ca2+]j While an increase in [Ca2+]i increases oxidative stress in neurons.

収録刊行物

  • Jpn.J.Pharmacol.

    Jpn.J.Pharmacol. 72 (4), 381-385, 1996

    公益社団法人 日本薬理学会

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