Suppression by Azelastine Hydrochloride of NF-κB Activation Involved in Generation of Cytokines and Nitric Oxide

• Yoneda Kazunori

  Department of Oral Surgery, Kochi Medical School

• Yamamoto Tetsuya

  Department of Oral Surgery, Kochi Medical School

• Ueta Eisaku

  Department of Oral Surgery, Kochi Medical School

• Osaki Tokio

  Department of Oral Surgery, Kochi Medical School

書誌事項

タイトル別名

• Suppression by Azelastine Hydrochloride of NF-/.KAPPA.B Activation Involved in Generation of Cytokines and Nitric Oxide.
• Suppression by Azelastine Hydrochloride

抄録

The influence of the anti-allergy agent azelastine hydrochloride (Azeptin®)on NF-κB activation associated with the. generation of cytokines and nitric oxide (NO)was investigated in various kinds of human and mouse cells. Azeptin dose-dependently suppressed both DNA and protein synthesis in human gingival fibroblasts (HF)and also suppressed blastogenesis of human peripheral blood lymphocytes (PBL). Generation of tumor necrosis factor-alpha, interleukin 1-beta, granulocyte-macrophage colony-stimulating factor and interleukin-6 from 10^-5 M Azeptin-treated PBL and human monocytes (HM)was decreased to approximately 1/3 to 2/3 of the control levels. In parallel with the decreased cytokine generation, each cytokine mRNA was less expressed in the presence of 10^-5 M Azeptin. In addition, both inducible nitric oxide synthase-mRNA level and NO generation in mouse peritoneal macrophages were suppressed by 10^-5 M Azeptin. Being compatible with those results, Azeptin (10^-5 M)suppressed activation of NF-κB in PBL, HM and HF. These results appear to indicate that suppression of cytokine and NO generation by Azeptin results at least partially from the inhibition of NF-κB activation.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 73 (2), 145-153, 1997

  公益社団法人 日本薬理学会

キーワード

• Azelastine hydrochloride
• Cytokine
• Nitric oxide synthase
• NF-κB

詳細情報

• CRID: 1390001204286962048
• NII論文ID: 10008677145
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.73.145
• COI: 1:CAS:528:DyaK2sXhsVWnu7k%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4153689
• PubMed: 9074948
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4153689
• Web Site: https://search.jamas.or.jp/link/ui/1997124967
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可