Muscarinic Acetylcholine Receptors on Rat Thymocytes:Their Possible Involvement in DNA Fragmentation

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Several studies have shown that the nervous (and hormonal)system controls immune functions. In the present study, we examined the presence of muscarinic acetylcholine receptors and the effect of carbachol on DNA fragmentation in adult rat thymocytes. Rat thymocytes possessed high affinity binding sites for the muscarinic antagonist [<SUP>3</SUP>H]3-quinuclidinyl benzilate (QNB). The average number of binding sites per cells was 3000, and the equilibrium dissociation constant of [3H]QNB on intact cell was approximately 80 nM. The binding was inhibited by an Ml and M3-selective antagonist, 4-diphenylacetoxy-<I>N</I>-methylpiperidine methiodine (4-DAMP). Hydrocortisone (100 mg/kg, s.c.)treatment of rats for 2 days prior to sacrifice increased the average number of [<SUP>3</SUP>H]QNB binding sites on thymocytes by 82±33%. The gel electrophoresis of DNA extracted from carbachol-treated thymocytes revealed a ladder pattern typical of intranucleosomal fragmentation. The addition of oxotremorine-M also induced DNA fragmentation and the effects of muscarinic agonists were inhibited by the addition of atropine or 4-DAMP. The results suggest the existence of muscarinic receptors and the possible involvement in apoptosis in thymocytes.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 73(4), 311-316, 1997-04-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008677774
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4196927
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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