Phorbol 12-Myristate 13-Acetate(PMA)-Induced Oxyradical Production in Rheumatoid Cells

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抄録

We successfully detected the oxyradical production in human synovial A (macrophage-like)and B (fibroblast-like)cells by phorbol 12-myristate 13-acetate (PMA)using the luminol-chemiluminescence method. The PMA (0.1 μg/ml)-induced photon generation was abolished by an O<SUB>2</SUB><SUP>-</SUP> scavenger, superoxide dismutase, and an H<SUB>2</SUB>0<SUB>2</SUB> scavenger, catalase, suggesting that the stimulus produced oxyradicals in synovial cells. Both of these responses were abolished by a protein kinase C (PKC)inhibitor, calphostine C, but unaffected by an intracellular Ca<SUP>2+</SUP> chelator, BAPTA-AM, and Ca<SUP>2+</SUP> removal from the extracellular medium. These findings suggest that synovial A and B cells produce oxyradicals through PKC-mediated and [Ca<SUP>2+</SUP>]<SUB>i</SUB>-independent mechanisms, probably through the activation of NADPH oxidase.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 73(4), 347-351, 1997-04-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008677891
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    00215198
  • NDL 記事登録ID
    4196932
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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