Modulation of Tyrosine Kinase Activity Has Multiple Actions on Insulin Release from the Pancreatic β-Cell:Studies with Lavendustin A

• Hisatomi Mitsuro

  Second Department of Internal Medicine, Nagoya University School of Medicine

• Hayakawa Tetsuo

  Second Department of Internal Medicine, Nagoya University School of Medicine

• Hidaka Hiroyoshi

  Department of Pharmacology, Nagoya University School of Medicine

• Niki Ichiro

  Department of Pharmacology, Nagoya University School of Medicine

書誌事項

タイトル別名

• Modulation of Tyrosine Kinase Activity Has Multiple Actions on Insulin Release from the Pancreatic .BETA.-Cell: Studies with Lavendustin A.
• Modulation of Tyrosine Kinase Activity

抄録

We investigated the role of tyrosine kinases in the regulation of insulin release from a hamster β-cell line, HIT T15, using selective tyrosine kinase inhibitors. Genistein increased the insulin release induced by glucose, but herbimycin A, tyrphostins and the erbstatin analogue failed to change the release. Lavendustin A at 0.1 nM -1 μM caused a concave-shaped inhibition of the insulin release stimulated by 7 mM glucose. The inhibitory effect of lavendustin A was overcome by higher concentrations of glucose. Lavendustin B, the negative control analogue, had no effect on the release. Lavendustin A at a nanomolar range progressively inhibited insulin release by high K⁺ (50 mM)-depolarization, whereas the inhibitor did not change the insulin release by Ca²⁺ ionophore (A23187). On the contrary, lavendustin A at 10 nM significantly increased insulin release when glucose-induced insulin release was enhanced by either 5 μM forskolin or 162 nM 12-Ο-tetradecanoylphorbol 13-acetate. Lavendustin A failed to influence the Ca²⁺-induced insulin release from HIT cells permeabilized with streptolysin-Ο. These findings suggest that tyrosine kinases may play versatile roles in the control of insulin release from the pancreatic β-cell.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 74 (2), 203-208, 1997

  公益社団法人 日本薬理学会

キーワード

• Insulin release
• Tyrosine kinase
• Pancreatic β-cell
• Lavendustin A
• yrosine kinase inhibitor

詳細情報

• CRID: 1390001204286283136
• NII論文ID: 10008679009
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.74.203
• COI: 1:CAS:528:DyaK2sXktVOgtr8%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4260350
• PubMed: 9243329
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4260350
• Web Site: https://search.jamas.or.jp/link/ui/1997232445
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可