Role of Cellular Na+ Accumulation in Acetylcholine-Induced Desensitization of Guinea Pig Ileal Longitudinal Muscle.

• Horio Shuhei

  Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima

• Moritoki Hideki

  Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima

書誌事項

タイトル別名

• Role of Cellular Na+Accumulation in Ace

抄録

The role of cellular Na⁺ accumulation in acetylcholine-induced desensitization was investigated in guinea pig ileal longitudinal muscle. Desensitization was induced by the pretreatment with acetylcholine (10^-4 M, 30 min)and was expressed by the rightward shift in the concentration-response curve for acetylcholine after the treatment. The same treatment with acetylcholine caused accumulation of cellular Na⁺ that amounted to about 3.5-fold of the control level. To study the relationship between the gain of cellular Na⁺ and the development of desensitization, we treated the muscle strips with acetylcholine under the condition in which the external Na⁺ concentration ranged from zero to 149.2 mM. The result showed that cellular Na⁺ content is closely related to the extent of desensitization; that is, desensitization was at the lowest level when acetylcholine induced no increase in cellular Na⁺, while desensitization developed in proportion to the increase in cellular Na⁺ content. However, when cellular Na⁺ was increased by another method (by the treatment with ouabain), the inhibition of the acetylcholine response was far less than that observed in the case of desensitization. We concluded that both muscarinic stimulation and the accompanying accumulation of cellular Na⁺ are required for desensitization to occur in full. This desensitization could be the result of a muscarinic stimulated and cellular Na⁺-dependent mechanism.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 74 (4), 291-295, 1997

  公益社団法人 日本薬理学会

キーワード

• Ileum (guinea pig)
• Desensitization
• Acetylcholine
• Ca<SUP>2+</SUP> channel
• Cellular Na<SUP>+</SUP>

詳細情報

• CRID: 1390001204284904832
• NII論文ID: 10008679404
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.74.291
• COI: 1:CAS:528:DyaK2sXlsF2rsLY%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4287824
• PubMed: 9307324
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4287824
• Web Site: https://search.jamas.or.jp/link/ui/1998015162
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可