Effects of Sucralfate and Its Components on Acid- and Pepsin-Induced Damage to Rat Gastric Epithelial Cells

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We have established models of cell damage induced by acid and pepsin using rat gastric epithelial cells (RGMI). In the present study, the effects of aluminum hydroxide [AI(OH)<SUB>3</SUB>] and potassium sucrose octasulfate (KSOS), which are components of sucralfate, and sucralfate on cell damage and peptic activity of pepsin were examined. Pretreatment of cells with sucralfate (0.1-3 mg/ml) or Al(OH)<SUB>3</SUB> (0.1-1 mg/ml) for 2 hr prevented both acid (pH 4.0) and pepsin (pH 4.5) induced cell damage. However, KSOS (0.1-1 mg/ml) did not show any effects on two different types of cell damage. The peptic activity of pepsin at pH 4.5 was about 10010 of that at pH 2.0. Sucralfate and KSOS slightly inhibited peptic activity at pH 4.5. Al(OH)<SUB>3</SUB> inhibited peptic activity by approximately 50010; however, no concentration-dependent pattern was observed. Pepstatin (0.003-0.1 mg/ml), a specific inhibitor of pepsin, inhibited the peptic activity in a concentration-dependent manner. Here, we confirmed that sucralfate and Al(OH)3 have cytoprotective effects against acid and pepsin-induced cell damage. The mechanism behind the cytoprotective effects of sucralfate seems to relate to adhesion of the cell surface and neutralization of hydrogen ion by aluminum that prevents the penetration of hydrogen ions into the cells.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 75(1), 21-25, 1997-09-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008679637
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4307641
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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