Calcium Oscillations in Single Cultured Chinese Hamster Ovary Cells Stably Transfected with a Cloned Human Cholecystokinin (CCK)_B Receptor

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In Chinese hamster ovary cells stably expressing the cloned human cholecystokinin (CCK)<SUB>B</SUB> /gastrin receptor, cholecystokinin octapeptide (CCK-8) evoked increases in [Ca<SUP>2+</SUP>]<SUB>i</SUB> monitored by digitized video imaging of fura-2 fluorescence ratios. At concentrations around 10 pM, CCK-8 elicited [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillations, which were blocked by elimination of extracellular Ca<SUP>2+</SUP>, by a phospholipase C inhibitor, U-73122, by a protein kinase C inhibitor, H7, as well as by phospholipase A2 (PLA<SUB>2</SUB>) inhibitors, ONO-RS-082 and aristolochic acid. At higher concentrations, CCK-8 induced a single biphasic [Ca<SUP>2+</SUP>]<SUB>i</SUB> rise consisting of a large peak followed by a lower sustained plateau, while the response turned into [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillation when the extracellular Ca<SUP>2+</SUP> was eliminated or a PLA<SUB>2</SUB> inhibitor was included. CCK-8 stimulated the release of arachidonic acid, and this was inhibited by aristolochic acid. Arachidonic acid caused an increase in [Ca<SUP>2+</SUP>]<SUB>i</SUB> which was dependent upon extracellular Ca<SUP>2+</SUP>. These results suggest that the activation of PLA<SUB>2</SUB> might be involved, at least in part, in the Ca<SUP>2+</SUP> influx that maintains the sustained plateau phase of [Ca<SUP>2+</SUP>]<SUB>i</SUB> as well as the [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillation when CCK<SUB>B</SUB> receptors are stimulated.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 75(1), 33-42, 1997-09-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008679679
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4307643
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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