Requirement of Cytokines for Augmentation of the Antigen-Specific Antibody Responses by Ascorbate in Cultured Murine T-Cell-Depleted Splenocytes.

• Mitsuzumi Hitoshi

  Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

• Kusamiya Makoto

  Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

• Kurimoto Takafumi

  Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

• Yamamoto Itaru

  Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

書誌事項

タイトル別名

• Requirement of Cytokines for Augmentati

抄録

To gain a better understanding of the possible mechanisms by which a stable form of ascorbate, ascorbic acid 2-glucoside (AA-2G), as an ascorbate source, augments antibody responses, we examined whether AA-2G enhances the anti-sheep-red-blood-cell (SRBC) plaque-forming cell (PFC) responses elicited with distinct interleukins that provide signals for B-cell proliferation and differentiation in cultured murine T-cell-depleted splenocytes. The anti-SRBC PFC responses were markedly reduced by T-cell depletion; and additions of the concanavalin A-stimulated murine splenocytes supernatant (CAS) or interleukin (IL)-1β, IL-2, IL-5, IL-4 or IL-6 to the culture limitedly restored the immune responses. AA-2G synergistically stimulated the anti-SRBC PFC responses in the presence of IL-1β-, IL-2, IL-5 or CAS, IL-1β among these cytokines being most highly affected. However, it failed to enhance the PFC responses elicited by IL-4 or IL-6. Repeated additions of ascorbic acid (AsA) during experimental periods could also produced the enhancing effect, but a single addition of the vitamin did not, because of its instability in the medium. It was shown that exposure to IL-1β, IL-2 or IL-5 must be done at early times after antigen stimulation of the cells to support their optimal responses and that AsA exerted its effect on day 2 and day 3 after the start of culture. These results suggest that AsA may up-regulate the in vitro IgM antibody responses in a cytokine-dependent manner.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 78 (2), 169-179, 1998

  公益社団法人 日本薬理学会

キーワード

• Ascorbic acid
• Ascorbic acid 2-glucoside
• Antigen-specific antibody response
• Interleukin
• T-cell-depleted splenocyte

詳細情報

• CRID: 1390001204287076480
• NII論文ID: 10008680697
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.78.169
• COI: 1:CAS:528:DyaK1cXmvFyqs7g%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4591514
• PubMed: 9829620
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4591514
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819310455?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819310455?httpAccept=text/plain
• Web Site: https://www.jstage.jst.go.jp/article/jjp/78/2/78_2_169/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999065775
• 本文言語コード: en
• データソース種別:
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