Bacterial Expression and Functional Characterization of a Rat Thyroid Hormone Sulfotransferase, ST1B1

この論文にアクセスする

この論文をさがす

著者

    • SHIMADA Miki
    • Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University
    • YAMAZOE Yasushi
    • Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

抄録

At least three forms of phenol sulfotransferase (ST) ST1B1, ST1A1 and ST1C1 are contained in rat livers. To identify the form contributing to the metabolism of 3, 3′, 5-triiodothyronine (T<SUB>3</SUB>), functional characterization of these forms was performed by expression in <I>Escherichia coli</I>. ST1B1 and ST1C1 were shown to be active on sulfation towards T<SUB>3</SUB> with high affinity (K<SUB>m</SUB>: 44.4 and 25.8 μM, respectively), whereas ST1A1 had low affinity. In Western blotting using antibodies raised against the individual ST, hepatic contents of each ST were quantitatively determined. ST1B1 showed no clear sex-difference, whereas the level of ST1C1 was higher in adult males than adult females. The content of ST1B1 was 1.4, 6.8 and 10 times higher than that of ST1C1 in adult males, adult females and both sexes of immature rats, respectively. The developmental pattern of ST1B1 was similar to that of ST1A1, but differed from that of ST1C1. These results indicate that ST1B1 and ST1C1 are involved in T<SUB>3</SUB> metabolism in rats and ST1B1 is the constitutive form across sexes and ages.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 79(4), 467-475, 1999-04-01

    The Japanese Pharmacological Society

参考文献:  32件中 1-32件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008683558
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4706136
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ