Bacterial Expression and Functional Characterization of a Rat Thyroid Hormone Sulfotransferase, ST1B1.

• Fujita Ken-ichi

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

• Nagata Kiyoshi

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

• Watanabe Eriko

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

• Shimada Miki

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

• Yamazoe Yasushi

  Division of Drug Metabolism and Molecular Toxicology, Faculty of Pharmaceutical Sciences, Tohoku University

Abstract

At least three forms of phenol sulfotransferase (ST) ST1B1, ST1A1 and ST1C1 are contained in rat livers. To identify the form contributing to the metabolism of 3, 3′, 5-triiodothyronine (T₃), functional characterization of these forms was performed by expression in Escherichia coli. ST1B1 and ST1C1 were shown to be active on sulfation towards T₃ with high affinity (K_m: 44.4 and 25.8 μM, respectively), whereas ST1A1 had low affinity. In Western blotting using antibodies raised against the individual ST, hepatic contents of each ST were quantitatively determined. ST1B1 showed no clear sex-difference, whereas the level of ST1C1 was higher in adult males than adult females. The content of ST1B1 was 1.4, 6.8 and 10 times higher than that of ST1C1 in adult males, adult females and both sexes of immature rats, respectively. The developmental pattern of ST1B1 was similar to that of ST1A1, but differed from that of ST1C1. These results indicate that ST1B1 and ST1C1 are involved in T₃ metabolism in rats and ST1B1 is the constitutive form across sexes and ages.

Journal

• The Japanese Journal of Pharmacology

  The Japanese Journal of Pharmacology 79 (4), 467-475, 1999

  The Japanese Pharmacological Society

Keywords

• <I>Escherichia coli</I>
• Expression system
• Histidine-tag
• Phenol sulfotransferase
• Thyroid hormone

Details

• CRID: 1390001204287416960
• NII Article ID: 10008683558
• NII Book ID: AA00691188
• DOI: 10.1254/jjp.79.467
• COI: 1:CAS:528:DyaK1MXislejur4%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL BIB ID: 4706136
• PubMed: 10361887
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4706136
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819309291?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819309291?httpAccept=text/plain
• Web Site: https://www.jstage.jst.go.jp/article/jjp/79/4/79_4_467/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999202071
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed