Soluble Proteins from <I>Staphylococcus aureus</I> can Change Expression of CD11b and CD62L, but Not H<SUB>2</SUB>O<SUB>2</SUB>Production by Human Blood Granulocytes

• ONOGAWA Tsuyoshi

  Department of Immunology, School of Health Sciences, Kyorin University

• ENDO Nobuko

  Department of Immunology, School of Health Sciences, Kyorin University

Bibliographic Information

Other Title

• ヒト末梢血顆粒球CD11b, CD62Lの変化およびH<SUB>2</SUB>O<SUB>2</SUB>産生に及ぼす<I>Staphylococcus aureus</I> 可溶性菌体成分の影響
• ヒト末梢血顆粒球CD11b,CD62Lの変化およびH2O2産生に及ぼすStaphylococcus aureus可溶性菌体成分の影響
• ヒト マッショウケツ カリュウキュウ CD11b CD62L ノ ヘンカ オヨビ H2O2 サンセイ ニ オヨボス Staphylococcus aureus カヨウセイ キンタイ セイブン ノ エイキョウ
• Soluble Proteins from Staphylococcus aureus can Change Expression of CD11b and CD62L, but Not H2O2Production by Human Blood Granulocytes

Abstract

We examined the production of CD11b, CD62L and H₂0₂by human peripheral blood granulocytes after treatment with soluble proteins prepared from five different pressure-disrupted strains of Staphylococcus aureus (SaSP) by flow cytometory. Peripheral blood was treated with final SaSP concentrations of 0.05, 0.5and 5.0μg for 20 min at 37°C. The ratio of CD11b positive granulocytes did not increase at concentrations from 0.05 to 5.0μg, but fluorescence intensity showed about two and threefold increase, respectively, at concentrations of 0.5 and 5.0μg, in comparison with that of control cells. The ratio CD62L positive cells decreased as follows: 0.5μg, 53.8% and 5.0μg, 19.0%, respectively, whereas the control value was 79.8%. Fluorescence intensity also decreased as follows: 0.5μg, 10.4 and 5.μEg, 92, respectively, whereas the control value was 46.8. Slight induction of H₂0₂ was found at 5.0μg concentration only. In addition, SaSP treatment granulocytes that stimulated with PMA (1 ng) increased H₂0₂production. Thus, SaSP has no beneficial effect against H₂0₂production by granulocytes. All of the SaSP preparations indicated similar results for the production of CD11b, CD62L and H₂0₂by granulocytes. SaSP effects activation of granulocytes, and the activation may occur independently of protein kinase C.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 73 (4), 328-335, 1999

  The Japanese Association for Infectious Diseases

Keywords

• Staphylococcus aureus
• CD11b
• CD62L
• H<SUB>2</SUB>O<SUB>2</SUB>

Details

• CRID: 1390282680027491584
• NII Article ID: 130004330761
• NII Article ID: 10008715562
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.73.328
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4718402
• PubMed: 10356890
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4718402
• Web Site: https://search.jamas.or.jp/link/ui/1999227451
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed