Evaluation of Urinary Antigen Detection Methods for Rapid Diagnosis of <I>Legionella</I> pneumonia

• ARAKAKI Noriko

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

• HIGA Futoshi

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

• KOIDE Michio

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

• TAKEYAMA Masao

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

• SHINZATO Takashi

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

• KUSANO Nobuchika

  Clinical Laboratory, Ryukyu Uneversity Hospital

• YABUUCHI Eiko

  Department of Microbiology and Immunology, Aichi Medical University

• SAITO Atushi

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyu's

Bibliographic Information

Other Title

• レジオネラ肺炎に対する早期診断法としての尿中抗原検出法の意義
• レジオネラ ハイエン ニ タイスル ソウキ シンダンホウ ト シテ ノ ニョウ チュウ コウゲン ケンシュツホウ ノ イギ
• Evaluation of Urinary Antigen Detection Methods for Rapid Diagnosis of Legionella pneumonia

Abstract

We have evaluated urine specimens of presumptive cases of legionnaires' disease (110 cases, 173 sample), collected in the past eight years (April, 1990-August, 1998) with the Binax EIA kit which detects the soluble antigen of Legionella pneumophila serogroup (SG) 1, and the Biotest EIA kit which detects Legionella species.<BR>Seven cases (19 specimens) were positive for the Binax EIA kit, and nine cases (22 specimens) were positive for the Biotest EIA kit. The sensitivity for culture, PCR, IFA method were 100%, 100%, and 50%, the specificity for these method were 93%, 97.1%, and 90% respectively. Overall agreements for these method were 93.5%, 97.4%, 86.8%, these results suggested that the urinary antigen detection test had high sensitivity and specificity. Our study indicated that concentrated urine samples increase sensitivity.<BR>We also evaluated the capabilities of both EIAs to detect soluble antigens were extracted from bacterial suspension of 18 strains of 5 Legionella species by heating. Both assays detected L. pneumophila serogroups 1 to 14, L. bozemanii. The Binax EIA proved to be useful as the Biotest EIA for diagnosis of legionellosis caused by Legionella species and serogroups other than L. pneumophila serogroup 1. Some cases have been shown to excrete antigen for prolonged period of times despite recovery from infection, so that the patient's history should be sought.<BR>The urine antigen detection EIA methods proved to be rapid and easy to use, detect antigen in the early stage of the desease with high sensitivity and specificity. Its use for the definition of legionellosis should be considered in Japan.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 73 (5), 421-428, 1999

  The Japanese Association for Infectious Diseases

Keywords

• Legionella
• urinary antigen
• enzymeimmunoassay (EIA)
• rapid diagnosis

Details

• CRID: 1390282680026472704
• NII Article ID: 130004330770
• NII Article ID: 10008715672
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.73.421
• COI: 1:STN:280:DyaK1MzhtlOquw%3D%3D
• ISSN: 1884569X
• ISSN: 03875911
• ISSN: http://id.crossref.org/issn/03875911
• NDL BIB ID: 4737986
• PubMed: 10386021
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4737986
• Web Site: https://search.jamas.or.jp/link/ui/1999244717
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed