Evaluation of CO<SUB>2</SUB> Condition for Antibiotic Susceptibility Test of <I>Helicobacter pylori</I> on Microdilution Broth

• UDAGAWA Hirokazu

  Otsuka Tokyo Assay Laboratories

• SHISHIDO Hideki

  Otsuka Tokyo Assay Laboratories

• ITOH Takeshi

  Tokyo Kenbikyoin

• SHINGAKI Masao

  Tokyo Metropolitan Research Laboratory for Public Health

• HOSHIYA Satoshi

  Third Department of Internal Medicine, Kyorin University, School of Medicine

• TAKAHASHI Shinichi

  Third Department of Internal Medicine, Kyorin University, School of Medicine

Bibliographic Information

Other Title

• マイクロプレートを用いた微量液体希釈法での<I>Helicobacter pylori</I>薬剤感受性試験におけるCO<SUB>2</SUB>培養条件の検討
• マイクロプレートを用いた微量液体希釈法でのHelicobacter pylori薬剤感受性試験におけるC02培養条件の検討
• マイクロ プレート オ モチイタ ビリョウ エキタイ キシャクホウ デ ノ Helicobacter pylori ヤクザイ カンジュセイ シケン ニ オケル C02 バイヨウ ジョウケン ノ ケントウ
• Evaluation of CO2 Condition for Antibiotic Susceptibility Test of Helicobacter pylori on Microdilution Broth

Abstract

We determined the MICs of clarithromycin for 32 Helicobacter pylori strains isolated from gastric mucosa of patients, by using microdilution broth method under different microaerobic conditions (CO₂: 5% and 15%). The pH of broth was more acidic at 15% CO₂than that of at 5% CO₂. The MICs of clarithromycin for all H. pylori strains at15% CO₂ condition werehigher than that of clarithromycin at 5% CO₂, condition. Comparing the MICs with the results of eradication therapy, we concluded that the measurment of MIC under 5% CO₂, condition is more useful for expecting the clinical outcome.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 73 (6), 565-569, 1999

  The Japanese Association for Infectious Diseases

Keywords

• Hclicobacter pylori
• growth condition
• microdilution broth method
• susceptibility test

Details

• CRID: 1390282680027383680
• NII Article ID: 130004330781
• NII Article ID: 10008715854
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.73.565
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4773464
• PubMed: 10423946
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4773464
• Web Site: https://search.jamas.or.jp/link/ui/1999249082
• Data Source:
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed