Detection of Anti-<I>Chlamydia trachomatis</I> Antibody by Means of Enzyme Immunoassay Using Synthetic Peptide Antigen

• BANNAI Hisaichi

  Dept. of Medical Technology, Kyorin University School of Health Sciences

• KOMODA Teruko

  Dept. of Medical Technology, Kyorin University School of Health Sciences

• AKITA Hironobu

  Dept. of Pediatrics, St. Marianna University, Yokohama City Seibu Hospital

• IWATA Satoshi

  Dept. of Pediatrics, Kasumigaura National Hospital

• SATO Yoshitake

  Dept. of Pediatrics, Ohta General Hospital

• SUNAKAWA Keisuke

  Dept. of Infectious Disease, Kitasato University, School of Medicine

Bibliographic Information

Other Title

• 合成ペプチド抗原を用いた抗<I>Chlamydia trachomatis</I>抗体の検出
• 合成ペプチド抗原を用いた抗Chlamydia trachomatis抗体の検出(第72回日本感染症学会総会学術講演会座長推薦論文)
• ゴウセイ ペプチド コウゲン オ モチイタ コウChlamydia trachomatis コウタイ ノ ケンシュツ ダイ72カイ ニホン カンセンショウ ガッカイ ソウカイ ガクジュツコウエンカイ ザチョウ スイセン ロンブン
• Detection of Anti-Chlamydia trachomatis Antibody by Means of Enzyme Immunoassay Using Synthetic Peptide Antigen

Abstract

Newly developed diagnostic kits for the detection of Anti-Chlamydia trachomatis, Peptide-Chlamvdia (LOY: Meiji Milk Products Co., Ltd., Tokyo; for IgG and IgA), were evaluated using the microimmunofluorescence assay (MIF) as the gold standard. These results were also compared to results of testing by Sero-IPALISA and immunoblot (I-B). Detection by LOY in based on enzyme immunoassay with synthetic peptides as the antigen. Thirty serum samples from pediatric patients and 130serum samples from gynecology patients were used. All26pediatric samples that were positive for Chlamydia pneumoniae IgG antibody tested negative with LOY, indicating that the presence of the antibody against C. pneumoniae did not affect the assay by LOY.<BR>For 90 gynecological samples, the total, the positive and the negative agreement rates for IgG were quite high; i. e. 87.8%, 90.0% and 70.0% (LOY vs MIF), 85.6%, 85.0% and 90.0% (Sero-IPALISA vs MIF), and 92.0%, 94.9% and 70.0% (I-B vs MIF), respectively. On the other hand, many cases of MIF (-) and LOY (+) discrepancy were seen in IgA detection. In order to better understand the basis for such disagreement. 34serum samples were collected from patients whose cervical samples were negative for the Chlamydia group antigen based on the assay with IDEIA-Chlamydia^®. They were then assayed by MIF and LOY. The total, the positive and the negative agreement rates for IgG were 91.2%, 100% and 90.9%, while the total and the negative agreement rates for IgA were 88.2% and 88.2% (there were no IgA positive cases). Furthermore, 6 serum samples (1case of MIF (+) LOY (+) and5cases of MIF (-) LOY (+)) were provided to determine whether LOY detects C. trachomatis specific IgA antibody. Increasing amounts of C. trachomatis serovar L2were added to the serum samples resulting in a progressive decrease in their reactivity in the LOY assay. These results lead us to speculate that LOY can reveal even low levels of C. trachomatis specific IgA antibody.<BR>In conclusion, LOY can be used as an useful kit for detecting C. trachomatis antibody.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 73 (7), 633-639, 1999

  The Japanese Association for Infectious Diseases

Keywords

• Chlamydia trachomatis
• peptide antigen
• enzyme immunoassay

Details

• CRID: 1390001205050681856
• NII Article ID: 130004112698
• NII Article ID: 10008716010
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.73.633
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4785927
• PubMed: 10481397
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4785927
• Web Site: https://search.jamas.or.jp/link/ui/2000000383
• Data Source:
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed