Inducibility of Tumor Necrosis Factor Alpha to Peritoneal Macrophages and Lethality to Mice of Clinical Isolates of <I>Staphylococcus aureus</I>

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  • ENDO Nobuko
    Department of Immunology, Kyorin University School of Health Sciences
  • ONOGAWA Tsuyoshi
    Department of Immunology, Kyorin University School of Health Sciences

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Other Title
  • 臨床材料より分離された黄色ブドウ球菌のTNFα誘導性と致死活性
  • リンショウ ザイリョウ ヨリ ブンリサレタ オウショクブドウキュウキン ノ T
  • Inducibility of Tumor Necrosis Factor Alpha to Peritoneal Macrophages and Lethality to Mice of Clinical Isolates of Staphylococcus aureus

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Abstract

When murine resident peritoneal macrophages were treated in vitro with overnight culture supernatants of clinical isolates of S. aureus (21 strains) (S. aureus-CS) for 4 hrs at 37°C in a 5% CO2-air humidified incubator, TNFα induction from the treated cells was observed in 20/21 strains. The amount of TNFα dispersed from 88.4 to 1726.5 pg/ml but more or less amount of TNFα did not relate with the presence of TSST-1 and enterotoxin production of S. aureus. Recombinant protein A induced TNFα production by macrophages dose-dependently, but the relationship between the amount of protein A in each S. aureus CS and that of TNFα induced from macrophages treated with them were not satisfactory (r =0.69). When each strain was injected (×109 bacterial cells) interperitonealy to mice, 13/21 strains indicated lethal activity. Relation between TNFα inducibility in vitro and lethal activity, however, was not found (r= 0.4). These results suggest that TNFα inducibility is the basic biological activity of S. aureus, although there is a difference in the induction amount and in component (s) to induce TNFα on each strain, and then because the strain is strong in TNFα indicibility, it does not necessarily follow that pathogenicity is strong.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (3), 231-237, 1998

    The Japanese Association for Infectious Diseases

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