Analysis of Transmission of Burkholderia cepacia Isolates in an Intrahospital by Randomly Amplified Polymorphic DNA-PCR Method

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  • OKAZAKI Mitsuhiro
    Department of Clinical Laboratories, Kyorin University Hospital
  • MORITA Koji
    Department of Microbiology, Kyorin University School of Health Sciences
  • KOCHI Naoko
    Department of Clinical Laboratories, Kyorin University Hospital
  • ARAKI Koji
    Department of Clinical Laboratories, Kyorin University Hospital
  • YOSHIZAWA Mie
    Trauma and Critical Care Center, Kyorin University Hospital
  • WADA Hiroshi
    Department of Microbiology, Kyorin University School of Health Sciences
  • SHIBATA Mikiyoshi
    Department of Microbiology, Kyorin University School of Health Sciences
  • WATANABE Noboru
    Department of Microbiology, Kyorin University School of Health Sciences
  • EGAMI Teruo
    Department of Clinical Laboratories, Kyorin University Hospital
  • FURUYA Nobushige
    Department of Clinical Laboratories, Kyorin University Hospital
  • KANAMORI Masato
    Department of Microbiology, Kyorin University School of Health Sciences
  • SHIMAZAKI Shuji
    Trauma and Critical Care Center, Kyorin University Hospital
  • UCHIMURA Hidemasa
    Department of Clinical Laboratories, Kyorin University Hospital

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Other Title
  • Randomly amplified polymorphic DNA-PCR法による<I>Burkholderia cepacia</I>の院内分離株の伝播の解析
  • Randomly amplified polymorphic DNA-PCR法によるBurkholderia cepaciaの院内分離株の伝播の解析
  • Randomly amplified polymorphic DNA-PCRホ

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Abstract

Strains of Burkholderia cepacia isolated in our hospital from November 1995 to September 1996 were classified with randomly amplified polymorphic DNA-PCR (RAPD-PCR) and conventional biochemical tests (ID test·NF-18, API2ONE, and Neg Combo 4J kit), and intrahospital isolates of B. cepacia were analysed. During the period 28 strains from inpatients and 2 from medical apparatus were isolated. Twenty four of 28 (85.7%) were from sputum. In 1996 from January to February, 20 strains were detected from 8 inpatients, and two strains were from the nebulizers at the Trauma and Critical Care Center (TCC). With typing of B. cepacia by conventional methods no epidemiological relations among isolates were found. However, DNA patterns of original isolates from the nebulizers at TCC by RAPD-PCR were identical with those of isolates in sputa from patients in other wards who had stayed at TCC, indicating that TCC was an initial source of transmission and the strain was transmitted with the patients to the wards.<BR>These results suggest that RAPD-PCR method might be an useful tool to analyse an epidemiological survey for intrahospital transmission of isolate.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (7), 688-693, 1998

    The Japanese Association for Infectious Diseases

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