<I>In Vitro</I> Specific Binding of Shiga Toxin 1 and 2 by TAK-751S (Gb3 analog)

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  • TAKEDA Tae
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • YOSHINO Ken-ichi
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • ADACHI Eriko
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • YAMAGATA Kyohko
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • UCHIDA Hiroshi
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • OKONOGI Kenji
    Pharmacology Laboratories, Pharmaceutical Research Division, TAKEDA Chemical Industries, Ltd.
  • IIZAWA Yuji
    Pharmacology Laboratories, Pharmaceutical Research Division, TAKEDA Chemical Industries, Ltd.

Bibliographic Information

Other Title
  • TAK-751S (Gb3アナログ) の志賀毒素吸着作用
  • TAK-75IS(Gb3アナログ)の志賀毒素吸着作用
  • TAK-75IS Gb3 アナログ ノ シガ ドクソ キュウチャク サヨウ
  • In Vitro Specific Binding of Shiga Toxin 1 and 2 by TAK-751S (Gb3 analog)

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Abstract

TAK-751S is a synthetic trisaccharide coupled to Chromosorb P using a spacer sequence of 8-methoxycarboyloctyl (MCO). Its chemical structure is similar to a human receptor (Gb3) of Stx produced by enterohemorrhagic Escherichia coli (EHEC). In vitro efficacy of TAK-715S was studied by using ACHN cultured cell assay, which is sensitive and specific for measuring low level of Stx.<BR>Under various conditions, TAK-751S was mixed with purified Stx1 and Stx2, and residual free toxins in the solution were measured by using ACHN cells. TAK-715S was demonstrated to bind specifically to Stx1 and Stx2 under the condition similar to a human intestine while Chromsorb P did not bind to any Stx. The binding activity was stable in the presence of various processed foods, fresh vegetables and fruits. Antibiotics such as fosfomycin, kanamycin and norfloxacin did not disturb its binding capability. Minimum inhibitory concentrations of these antibiotics against Staphylococcus aureus FDA209P or E. coli NIH J JC-2 neither changed after incubating with TAK-751S for 60 min at 37°C.<BR>These results suggest that TAK-751S can be given orally with various foods and antibiotics for the elimination of Stx1 and Stx2 in the gut of patients with EHEC infections.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (9), 924-934, 1998

    The Japanese Association for Infectious Diseases

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