Isolation of Legionella and Free-Living Amoebae at Hot Spring Spas in Kanagawa, Japan

• KUROKI Toshiro

  Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory

• YAGITA Kenji

  Department of Parasitology, National Institute of Infectious Diseases

• YABUUCHI Eiko

  Department of Microbiology and Immunology, Aichi Medical University

• AGATA Kunio

  Tsukuba Technical Research Center, Aquas Co. Ltd.

• ISHIMA Tomoki

  Tsukuba Technical Research Center, Aquas Co. Ltd.

• KATSUBE Yasuji

  Laboratory of Veterinary Public Health, College of Bioresouce Sciences, University of Nihon

• ENDO Takuro

  Department of Parasitology, National Institute of Infectious Diseases

Bibliographic Information

Other Title

• 神奈川県下の温泉浴槽水中における<I>Legionella</I>属菌と自由生活性アメーバ調査
• 神奈川県下の温泉浴槽水中におけるLegionella属菌と自由生活性アメーバ調査
• カナガワ ケンカ ノ オンセン ヨクソウ スイチュウ ニ オケル Legion

Abstract

Microbiological contamination of hot spring bath water is a public health concern. A province-wide survey was carried out to determine the extent and distribution of both Legionella and free-living amoebae contamination. Among 30 samples of hot spring bath from 12 sites in Kanagawa, Japan, L. pneumophila was detected in 21 water samples from 11 sites, ranging from 10¹-10³CFU/100ml. Serogroups 3, 5 and 6 of L. pneumophila were predominantly isolated from the samples. Naegleria (46.7%), _Platyamoeba (33.3%), Acanthamoeba (10.0%) and 2 other genera of free-living amoebae were detected in 22 samples from 11 sites. One or more genera of host amoebae of Legionella occurred in 17 samples (56.7%) from 9 sites. Another thing to be noted is that 13 water samples contained N. lovaniensis. Although N. lovaniensis is nonpathogenic, it is considered an indicator organism for places that are suitable for the growth of N. fowleri, a causative agent of primary amoebic meningoencephalitis in man.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 72 (10), 1050-1055, 1998

  The Japanese Association for Infectious Diseases

Keywords

• Legionella
• free-living amoebae
• host amoebae
• hot spring bath water
• water chemistry

Details

• CRID: 1390282680027599360
• NII Article ID: 130004112607
• NII Article ID: 10008719047
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.72.1050
• COI: 1:STN:280:DyaK1M%2Fmtl2ruw%3D%3D
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4590067
• PubMed: 9847523
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4590067
• Web Site: https://search.jamas.or.jp/link/ui/1999066704
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed