Effects of <I>Staphylococcus aureus</I> Protein A on the Production of Primary Host Defense Factors against <I>S. aureus</I> Infection

• ENDO Nobuko

  Department of Immunology, Kyorin University School of Health Sciences

• ONOGAWA Tsuyoshi

  Department of Immunology, Kyorin University School of Health Sciences

Bibliographic Information

Other Title

• 宿主の初期感染防御因子産生におよぼす黄色ブドウ球菌プロテインAの影響
• シュクシュ ノ ショキ カンセン ボウギョ インシ サンセイ ニ オヨボス オ
• Effects of Staphylococcus aureus Protein A on the Production of Primary Host Defense Factors against S. aureus Infection

Abstract

In vitro examination was carried out to investigate the effects of protein A of Staphylococcus aureus (S. aureus) on the production of fibronectin (Fn) and the third component of complement (C3) by macrophages and that of CD11b, CD49e and H₂O₂ by granulocytes. Fn production of cultured murine peritoneal macrophages (Mφ) increased significantly (P <0.01) by treatment for 4 hr at 37°C with recombinant PA (rPA) and the supernatant of overnight culture of S. aureus Cowan I strain (CoCS) (p<0.01), not that of Wood 46 strain (WoCS), in comparison with that of control. The activity of rPA was inhibited strongly in the presence of CYH (1.0μg/ml). The production of C3 by cultured Mφ did not increased by treatment for 4 hr at 37°C with rPA, CoCS and WoCS. In these cells treated with CoCS and WoCS, however, the production increased by cultivation in serum free medium for a further 20 hr at 37°C after the treatment. But increase was not found in rPA treated cells. On the other hand, the production of Mac-1, VLA-5 and H₂0₂ by granulocytes did not increase by treatment with rPA and CoCS. These results show that rPA and PA in CoCS are major components which stimulates Fn synthesis and secretion by cultured Mφ, some component (s) contained in the supernatant of overnight culture of S. aureus increased the production C3 by cultured Mφ, and the supernatant may not contain stimulators to induce production of CD49e, CD11b and H₂O₂. Increase of Fn production of Mφ by PA stimulation may play an important role in the primary host defense against S. aureus infection.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 71 (1), 39-45, 1997

  The Japanese Association for Infectious Diseases

Keywords

• <I>Staphylococcus aureus</I> Protein A
• murine peritoneal macrophages
• fibronectin
• the third component of complement
• primary host defense

Details

• CRID: 1390001205046728576
• NII Article ID: 130004330500
• NII Article ID: 10008715578
• NII Article ID: 10008719607
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.71.39
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4151867
• PubMed: 9132427
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4151867
• Web Site: https://search.jamas.or.jp/link/ui/1997146303
• Data Source:
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  • NDL
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed