Evaluation of the Assay Technique for Detection of Anti-Chlamydial IgA and IgG Antibodies in PID Patients

DOI Web Site PubMed 9 References
  • YASUDA Jinsuke
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • HIGASHI Yayoi
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • TADA Yoshihiro
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • FUJIWARA Yoichiro
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • KASHIWAGI Nobuhito
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • KASHIWAGI Tomohiro
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • YAMAMOTO Takao
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
  • HONJO Hideo
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine

Bibliographic Information

Other Title
  • クラミジア性Pelvic Inflammatory Diseases診断における簡便な抗体検査試薬の検討
  • クラミジアセイ Pelvic Inflammatory Diseases シン

Search this article

Abstract

The purpose of this study is to evaluate the usefulnes and limitation of Rapizyme CHLAMYDIA ®, enzyme-linked immunosorbent assay (ELISA) for qualitative detection of anti-chlamydial IgG and IgA antibodies, in the serum of 92 PID patients and 73 pregnant women, compared with those of Sero IPALISA CHLAMYDIA ®.<BR>The result of Rapizyme analysis was obtained within 10 minutes with no special devices. Overall agreements of Rapizyme and Sero IPALISA were 90.9%(IgG) and 90.3%(IgA) in the total patients, 88.0%(IgG) and 85.9%(IgA) in PID patients, and 94.5%(IgG) and 95.9%(IgA) in pregnent women. The positive rate of Chlamydia in PID was 17.4%(16/92). Positive agreement of Rapizyme in Chlamydia positive PID and pregnant women was 100% in both IgG and IgA, and negative agreement was also 100%. Positive agreement in Chlamydia negative PID was 100% in both IgG and IgA, and negative agreement was 90.0%(IgG) and 83.3%(IgA). The results of Rapizyme were in close agreement with those of Sero IPALISA.<BR>COI (cut off index) of Sero IPALISA clearly decreased in 3 of 6 PID patients during a 3 to 6 months period after chemotherapy, but those changes were not observed in Rapizyme.<BR>These results suggest that Rapizyme CHLAMYDIA is a useful diagnostic kit for Chlamydial PID of outpatients.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 71 (3), 241-247, 1997

    The Japanese Association for Infectious Diseases

References(9)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top