Anti-bacterial Activities of a Sulfonated Human Immunoglobulin Preparation against Penicillin Resistant <I>Streptococcus pneumoniae</I> and <I>Pseudomonas aeruginosa</I>

DOI Web Site PubMed 1 Citations 6 References

Bibliographic Information

Other Title
  • スルホ化免疫グロブリン製剤のペニシリン耐性肺炎球菌および緑膿菌に対する抗菌活性
  • スルホカ メンエキ グロブリン セイザイ ノ ペニシリン タイセイ ハイエンキ
  • Anti-bacterial Activities of a Sulfonated Human Immunoglobulin Preparation against Penicillin Resistant Streptococcus pneumoniae and Pseudomonas aeruginosa

Search this article

Abstract

Anti-bacterial activities of a sulfonated human immunoglobulin preparation against penicillin resistant Streptococcus pneumoniae and Pseudomonas aeruginosa were examined. Five week old CBA/J mice were challenged by 10 times of 50% lethal doses of penicillin sensitive (SP1) and resistant (SP2) strains of Streptopcoccus pneuminiae serotype 19, and were treated with a sulfonated human immunoglobulin preparation (hIg). Fifty % protective dose (ED50) were 2-4mg hIg/ mouse. These doses were parallel with the challenged doses despite of the challenged bacteria, and it was calculated that 6×106 bacteria were killed by one mg of hIg in both bacteria.<BR>Minimal inhibitory concentrations (MIC) of piperacillin, flomoxef, imipenem, amikacin and ofloxacin against SP1 and SP2 were estimated under the presence of various concentrations of hIg. It was found that under the presence of over 10-20 mg/ml of hIg, SP1 and SP2 were not grown even without any antobiotic. That is, MIC of hIg itself against penicillin sensitive and resistant Streptococcus pneumoniae(HR) serotype 19 were 10-20 mg/ml. Since about 106 bacteria/ml were used for this test, it was calculated that 5-10×104 bacteria were killed by one mg of hIgin vitro. This result suggested that in vivo anti-bacterial activities of hIg could be 100 times higher than that in vitro. Synergistic or at least additive effects between hIg and all antibiotics tested were seen by the MIC. If hIg was 100 times effective in in vivo, these results suggested that hIg could improve the effect of chemotherapy in clinical cases.<BR>Similar in vitro test were carried out for Pseudomonas aeruginosa, and synergistic or at least additive effects between hIg and all antibotics tested were also confirmed in Pseudomonas aeruginosa. This result suggested that hIg could be effective for clinical pseudomonas infection.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 71 (8), 738-744, 1997

    The Japanese Association for Infectious Diseases

Citations (1)*help

See more

References(6)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top