Clinical Evaluation of a Reagent for Detection of DNA of <I>Mycobacterium tuberculosis</I> Complex Using the Ligase Chain Reaction (LCR) Method

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  • KOGA Hironobu
    The Second Department of Internal Medicine, Nagasaki University School of Medicine
  • KOHNO Shigeru
    The Second Department of Internal Medicine, Nagasaki University School of Medicine
  • TOMONO Kazunori
    The Second Department of Internal Medicine, Nagasaki University School of Medicine
  • NOHDA Kazuo
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • SUGAWARA Kazuyuki
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • HIRAKATA Yoichi
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • KAMIHIRA Shimeru
    Department of Laboratory Medicine, Nagasaki University School of Medicine
  • MIYASHITA Naoyuki
    Respiratory Diseases Division, Department of Internal Medicine, Kawasaki Medical School
  • MATSUSHIMA Toshiharu
    Respiratory Diseases Division, Department of Internal Medicine, Kawasaki Medical School
  • NISHINO Kazumi
    Respiratory Diseases Division, Department of Internal Medicine, National Sanitarium of Toneyama Hospital
  • YOKOTA Soichiro
    Respiratory Diseases Division, Department of Internal Medicine, National Sanitarium of Toneyama Hospital
  • KAWAMURA Yoshiaki
    Department of Microbiology, Gifu University School of Medicine
  • EZAKI Takayuki
    Department of Microbiology, Gifu University School of Medicine
  • WATANABE Masaharu
    Department of Laboratory Medicine, Chiba University School of Medicine
  • KANNO Harushige
    Department of Laboratory Medicine, Chiba University School of Medicine
  • YONEMITSU Hiroshi
    Department of Laboratory Medicine, Chiba University School of Medicine
  • OSUMI Mitsuhiko
    Respiratory Diseases Division, Department of Internal Medicine, National Sanitarium of Higashi-Saitama Hospital
  • TOYODA Takeo
    Respiratory Diseases Division, Department of Internal Medicine, National Sanitarium of Higashi-Saitama Hospital
  • AOYAGI Teruo
    Respiratory Diseases Division, Department of Internal Medicine, National Sanitarium of Higashi-Saitama Hospital

Bibliographic Information

Other Title
  • Ligase Chain Reaction (LCR) 法を用いた結核菌群DNA検出試薬の臨床的検討
  • Ligase Chain Reaction LCR ホウ オ モチイタ ケッカ
  • Clinical Evaluation of a Reagent for Detection of DNA of Mycobacterium tuberculosis Complex Using the Ligase Chain Reaction (LCR) Method

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Abstract

We evaluated the clinical efficacy of LCR MTB, a reagent developed by Abbott in the USA, in the full automatic ligase chain reaction (LCR) for detection of DNA of M. tuberculosis complex using a thermostable ligase.<BR>Using 458 samples isolated from patients with tuberculosis, LCR was compared with a smear method and with a culture method, and was also compared with two other methods of gene amplification, MTD and Amplicor, using 340 and 200 of the 458 samples, respectively.<BR>The LCR method detected M. tuberculosis in 49.8%(228/458) of the samples, and was superior to the smear method (31.9%, 146/458) and the culture method (39.1%, 179/458) in sensitivity. The LCR method was also superior to the MTD and Amplicor methods; sensitivity were 37.9%(129/340) for MTD vs. 47.6%(162/340) for LCR, and 56.5%(113/200) for Amplicor vs. 59.5%(119/200) for LCR. These favorable results and the convenience of the LCR method, which enables rapid detection of target genes with a high degree of sensitivity, strongly suggest that LCR MTB is useful as a reagent for detection of M. tuberculosis using nucleic acid amplification.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 71 (12), 1246-1251, 1997

    The Japanese Association for Infectious Diseases

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