尿路感染症の尿中における病原細菌の生態 Ecology of Pathogenic Bacteria in the Infected Urine

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著者

    • 大湾 知子 OWAN Tomoko
    • 琉球大学医学部細菌学講座 Department of Bacteriology,Faculty of Medicine,University of the Ryukyus

抄録

尿路病原菌が尿中で示す性状と分離したその菌が試験管内で示す性状とを比較検討した. 形態学的に見ると, 感染尿から直接回収した大腸菌は殆どが線毛を持たなかったがその菌を分離培養すると全ての菌が線毛を発現した. 尿中では英膜を持つ菌がしばしば見られたが, その菌を培養すると英膜は失われた. 溶血性大腸菌について見ると, 感染尿および尿中の菌から溶血素は検出されなかったが, 分離後の菌および培養上清からは溶血素が検出された. 菌体構成蛋白については外膜蛋白に著しい変化が見られた. 最も特徴的な変化は尿中の菌で見られた分子量約70kDaの蛋白が培養菌では消失していることであった. 濾過滅菌した健康人尿およびハートインフユージョンプロス (HIB) を培地として薬剤感受性を調べ, HIBにおける薬剤の最小発育阻止濃度 (MIC) を1とした時, 尿中におけるMICはオフロキサシン=25, アンピシリン=1, エリスロマイシン=3であった.

Ecological behaviors of uropathogenic bacteria in the infected urine were characterized, and they were compared to those <I>in vitro</I> cultivation. The organisms directly collected from the infected urine revealed no piliation, but when they were isolated and cultured in heart infusion broth (HIB), the pili appeared. The pili were identified as Type I pili as examined by immunogold electron microscopy. Although the organisms directly collected from the infected urine did not have pili and did not agglutinate against anti-Type I pili anti-serum, the whole cell lysate analysis by western blotting revealed that the organisms express Type I pili antigen intracellularly. The organisms in some cases revealed a capsule formation in the urine, but it disappeared when cultured <I>in vitro</I>. Hemolytic <I>Escherichia coli</I> were isolated from 9 cases out of 20 with acute cystitis, however, hemolysin was not detected from the infected urine and the organisms. When they were cultured in HIB, all strains produced the hemolysin. The composition of outer membrane proteins (OMPs) of the organisms in the infected urine was clearly different from those <I>in vitro</I> cultivation. As the most prominent finding, a 70 kDa OMP of the organisms in the infected urine disappeared <I>in vitro</I>. Antibacterial activities of several drugs examined in healthy human urine and in HIB were variable from drug to drug. The mean value of minimum inhibitory concentration (MIC) of ofloxasin was 25 times higher in the urine than in HIB, whereas the MIC of erythromycin was almost equal in both conditions.

収録刊行物

  • 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases

    感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases 70(7), 681-689, 1996-07-20

    The Japanese Association for Infectious Diseases

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各種コード

  • NII論文ID(NAID)
    10008723444
  • NII書誌ID(NCID)
    AN00047715
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    03875911
  • NDL 記事登録ID
    4005884
  • NDL 雑誌分類
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL 請求記号
    Z19-193
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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