Detection of DNA Specific for <I>Aspergillus</I> Species in Serum Samples from Two Patients with Invasive Pulmonary Aspergillosis

  • YAMAKAMI Yuriko
    The Second Department of Internal Medicine, Oita Medical University
  • HASHIMOTO Atsuro
    The Second Department of Internal Medicine, Oita Medical University
  • YAMAGATA Eiji
    The Second Department of Internal Medicine, Oita Medical University
  • NAGAOKA Hiroshi
    The Second Department of Internal Medicine, Oita Medical University
  • NAGAI Hiroyuki
    The Second Department of Internal Medicine, Oita Medical University
  • OHNO Eiji
    The Second Department of Internal Medicine, Oita Medical University
  • OTSUKA Eiichi
    The Second Department of Internal Medicine, Oita Medical University
  • KIKUCHI Hiroshi
    Blood Transfusion Service, Oita Medical University
  • NASU Masaru
    The Second Department of Internal Medicine, Oita Medical University

Bibliographic Information

Other Title
  • 侵襲性肺アスペルギルス症が強く疑われ, Nested PCR法で経過を観察し得た2症例の検討
  • Detection of DNA Specific for Aspergillus Species in Serum Samples from Two Patients with Invasive Pulmonary Aspergillosis

Search this article

Abstract

We investigated the possible presence of DNA specific for Aspergillus species in seurm samples of two patients who were strongly suspected for invasive pulmonary aspergillosis (IPA) by a nested polymerase chain reaction (PCR) method.<BR>Both patients were diagnosed as having acute myelogenous leukemia and treated with induction chemotherapy. During chemotherapy-induced granulocytopenia, they complained of high fever, and the chest X-rays indicated infiltration shadows in their lungs. They were treated with antibiotics intravenously, but no clinical improvement was observed. As the results of the nested PCR were positive at the acute stage of infection, amphotericin B i. v. and granulocyte colony stimulating factor s.c. administrations were started in both cases.<BR>In case 1, the infectious disease improved and the nested PCR results turned negative after treatment. In Case 2, in spite of the progression of the disease, the nested PCR results turned negative during treatment.<BR>Although we consider this method very useful for the diagnosis of IPA, further prospective evaluation with a large clinical population sample is required.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 70 (12), 1284-1289, 1996

    The Japanese Association for Infectious Diseases

Citations (1)*help

See more

References(15)*help

See more

Details

Report a problem

Back to top