Calcium Antagonist Inhibits Glomerular Cell Apoptosis and Injuries of L-NAME Exacerbated Nephrosclerosis in SHR.

DOI PubMed 被引用文献8件 参考文献28件
  • WATANABE Shigeko
    Department of Pathology, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine
  • ONO Hidehiko
    Department of Medicine, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine
  • ISHIMITSU Toshihiko
    Department of Medicine, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine
  • MATSUOKA Hiroaki
    Department of Medicine, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine
  • ONO Yuko
    Department of Pathology, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine
  • FUJIMORI Takahiro
    Department of Pathology, Division of Hypertension and Cardiorenal Disease, Dokkyo University School of Medicine

この論文をさがす

抄録

Increased apoptosis of glomerular cells, with progression of glomerulosclerosis, overactivity of the reninangiotensin system and elevation of glomerular pressure, follows chronic nitric oxide synthase (NOS) inhibition in spontaneously hypertensive rats (SHR). To gain insight into the regulation of glomerular cell apoptosis in severe nephrosclerosis, we investigated apoptosis, the expression of proliferative cell nuclear antigen (PCNA) in glomeruli, and glomerular morphometric changes in 20-week-old SHR, SHR treated with NOS inhibitor, NG-nitro-L-arginine methyl ester (L-NAME; 80mg/l in drinking water), and SHR treated with L-NAME and the calcium antagonist, efonidipine (20mg/kg per day), for 3 weeks. Apoptosis in non-sclerotic glomeruli was quantified by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling. The increase in systolic blood pressure and the severe proteinuria with severe nephrosclerosis induced by chronic NOS inhibition were completely prevented by efonidipine. Furthermore, the glomerular area and capillary tuft area were markedly increased in rats treated with efonidipine compared with both control rats (+30 and +42%, respectively, p<0.01) and rats treated with L-NAME (+35 and +56%, respectively, p< 0.01)-treated rats. This calcium antagonist also significantly inhibited the both increases of the glomerular cell apoptosis index (-72%) and the PCNA index (+44%), therefore the alteration between apoptosis and proliferation slightly increased the number of glomerular cells (subcapsular, +22%, p<0.01; juxtamedullary, +2%, not significant). Thus, the calcium antagonist efonidipine seems to play an important role in the regulation of apoptosis and proliferation of glomerular cells and may be effective in preventing nephrosclerosis exacerbated by NOS inhibition. (Hypertens Res 2000; 23: 683-691)

収録刊行物

被引用文献 (8)*注記

もっと見る

参考文献 (28)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ