Coordination Behavior of O2 and CO in a Solid Film Consisting of Hemoglobin and Maltose

• Joo Eun Chung

  Department of Polymer Chemistry, Waseda UniversityTokyo 169

• Hiromi Sakai

  Department of Polymer Chemistry, Waseda UniversityTokyo 169

• Shinji Takeoka

  Department of Polymer Chemistry, Waseda UniversityTokyo 169

• Hiroyuki Nishide

  Department of Polymer Chemistry, Waseda UniversityTokyo 169

• Eishun Tsuchida

  Department of Polymer Chemistry, Waseda UniversityTokyo 169

抄録

Solid hemoglobin (Hb) films were prepared by casting from a mixed solution of human hemoglobin and maltose (wt ratio of Hb/maltose is 60/40). The behavior of O2 and CO in coordinating to Hb in the films was then observed. The coordination equilibrium constants of both ligands were smaller by about an order of magnitude, and the rate constants (kon and koff) were smaller by about 13 orders of magnitude, than those of aqueous solutions of Hb. Interestingly, O2 and CO saturation was limited to 52% and 86%, respectively, under a partial pressure of 760 Torr (1 Torr = 133.322 Pa) of each gas, in spite of the one-to-one ligand binding ratio in aqueous solutions. This is due to the restraint of the structural change from a tense to a relaxed state in the solid phase. The limited saturation of the coordination in the solid phase was found to increase to 100% when a small amount (5.9%) of water was added; a significant structural change from the tense to the relaxed states followed.

収録刊行物

• Bulletin of the Chemical Society of Japan

  Bulletin of the Chemical Society of Japan 68 (3), 1006-1011, 1995-03-01

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360565169057546752
• NII論文ID: 10008888126
• NII書誌ID: AA00580132
• DOI: 10.1246/bcsj.68.1006
• ISSN: 13480634
• ISSN: 00092673
• Web Site: https://academic.oup.com/bcsj/article-pdf/68/3/1006/56192216/bcsj.68.1006.pdf
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