Enzyme Reactions in Microstructured Media. Subtilisin Catalysis in Alkyl Glucopyranosides Aggregates

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著者

    • KUNUGI Shigeru
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • HAYASHI Yuuji
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • KOYASU Akira
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • TANAKA Naoki
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • SHIRAISHI Makoto
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology

抄録

In order to understand the effect of the structures of the surrounding medium on biocatalytic reactions, hydrolytic catalysis by subtilisin Carlsberg in a ternary system containing octyl <i>β</i>-D-glucopyranoside (<i>β</i>-OG), water, and octanol (OcOH) was studied, with special reference to the enzyme reactivity and the morphology of the surfactant aggregates. <br>First, the effect of <i>β</i>-OG and other alkyl D-glucopyranosides (AG) on the subtilisin reaction was studied in the surfactant/water binary system; all four surfactants tested were found to inhibit the subtilisin catalysis. <br>Then, a detailed phase diagram of <i>β</i>-OG/water/octanol was determined and several liquid crystalline phases were found. <br>When the subtilisin reactions were measured in several compositions in the phase diagram, the maximum of the apparent rate was observed in reversed micells as well as in the reversed hexagonal phase at a certain [water]/[surfactant] ratio (<i>w</i><sub>o</sub>). The apparent optimal <i>w</i><sub>o</sub> was calculated to be around 5—6. These results were compared with the results in another ternary system of Aerosol OT/water/isooctane.

収録刊行物

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 68(3), 1012-1018, 1995-03-15

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10008888162
  • NII書誌ID(NCID)
    AA00580132
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00092673
  • データ提供元
    CJP書誌  J-STAGE 
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