Trichosanthes Japonica Agglutinin I Staining of Human Colonic Carcinoma : A Comparative Study Using Monoclonal Antibody Against Sialosyl-Tn Antigen

この論文にアクセスする

この論文をさがす

著者

抄録

<I>Trichosanthesjaponica agglutinin</I> I (TJA-I) having a high affinity to the NeuAcα2-6Galβ1-4GIcNAc terminal sequence was used for an immunohistochemical study to analyze the expression of the NeuAcα2-6Gal structure in human colonic tissues. A TJA-I binding substance was detected in the mucosa of colonic carcinoma, but not in the normal and transitional mucosa. The TJA-I staining was correlated with the degree of histo logical differentiation of the carcinoma. TJA-I preferentially stained well-differentiated and moderately differentiated adenocarcinoma. In the carcinoma containing mucin lakes, the TJA-I staining reaction was either weak in intensity or negative. A comparative study using monoclonal antibody against sialosyl-Tn antigen (NeuAcα2-6-GaINAc) known to be expressed in colonic carcinoma tissues revealed that the areas exhibiting strong expressions of siaosyl-Tn antigen separated from the areas vividly reactive for the TJA-I staining. In addition, the expression of sialosyl-Tn antigen, unlike the TJA-I staining, was strong in the mucinous areas of the carcinoma. The results obtained in the present study suggest the possibility that a comparative staining method with TJA-I and monoclonal antibody against sialosyl-Tn antigen could be helpful to the diagnosis of human colonic carcinoma.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 28(2), 155-162, 1995-04-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

参考文献:  29件中 1-29件 を表示

各種コード

  • NII論文ID(NAID)
    10008976369
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  J-STAGE 
ページトップへ