Histochemical Study of Helicobacter pylori and Surface Mucous Gel Layer in Various Gastric Lesions

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著者

    • AKAMATSU Taiji
    • The 2nd Department of Internal Medicine, Shinshu University School of Medicine
    • OTA Hiroyoshi
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • SHIMIZU Toshiki
    • The 2nd Department of Internal Medicine, Shinshu University School of Medicine
    • MATSUZAWA Kenji
    • The 2nd Department of Internal Medicine, Shinshu University School of Medicine
    • NAKAMURA Naoshi
    • The 2nd Department of Internal Medicine, Shinshu University School of Medicine
    • KIYOSAWA Kendo
    • The 2nd Department of Internal Medicine, Shinshu University School of Medicine

抄録

To elucidate the relation between <I>Helicobacter pylori (H. pylori)</I> and the surface mucous gel layer (SMGL), 32 cases (25 cases of gastric cancer and 7 of peptic ulcer) of surgically removed human stomach were examined by employing histochemical staining for mucins and <I>H. pylori</I>. This organism was demonstrated in 19 cases by histopathological and histochemical observation. All materials were fixed 2hr in cold Carnoy's solution to fix the SMGL and were embedded in paraffin. Serial paraffin sections were stained with galactose oxidase-cold thionin Schiff-paradoxical Concanavalin A staining (GOCTS-PCS). In addition, immunostaining with anti-<I>H. pylori</I> antibody was performed after the GOCTS-PCS sequence. <I>H. pylori</I> was observed not only on the apical surface of the surface mucous cells or between two lateral plasma membranes of adjacent surface mucous cells, but more abundantly in the SMGL. This organism was not found on the metaplastic cells showing intestinal properties, regenerated immature cells surrounding ulcerated lesions and neoplastic epithelial cells of carcinoma. In the SMGL, it tended to distribute in the layers of the surface mucous cell-type mucin, and, when it existed, marked disintegration of mucous layers and vacuolation was evident. These data suggest that disintegration of mucous in the SMGL is one of the factors which accelerate mucosal damage by this agent.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 28(2), 181-185, 1995-04-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

参考文献:  11件中 1-11件 を表示

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各種コード

  • NII論文ID(NAID)
    10008976474
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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