Histochemical Study of Helicobacter pylori and Surface Mucous Gel Layer in Various Gastric Lesions.

• Akamatsu Taiji

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Ota Hiroyoshi

  Department of Laboratory Medicine, Shinshu University School of Medicine

• Shimizu Toshiki

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Matsuzawa Kenji

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Fujimori Yoshifumi

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Nakamura Naoshi

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Kiyosawa Kendo

  The 2nd Department of Internal Medicine, Shinshu University School of Medicine

• Katsuyama Tsutomu

  Department of Laboratory Medicine, Shinshu University School of Medicine

抄録

To elucidate the relation between Helicobacter pylori (H. pylori) and the surface mucous gel layer (SMGL), 32 cases (25 cases of gastric cancer and 7 of peptic ulcer) of surgically removed human stomach were examined by employing histochemical staining for mucins and H. pylori. This organism was demonstrated in 19 cases by histopathological and histochemical observation. All materials were fixed 2hr in cold Carnoy's solution to fix the SMGL and were embedded in paraffin. Serial paraffin sections were stained with galactose oxidase-cold thionin Schiff-paradoxical Concanavalin A staining (GOCTS-PCS). In addition, immunostaining with anti-H. pylori antibody was performed after the GOCTS-PCS sequence. H. pylori was observed not only on the apical surface of the surface mucous cells or between two lateral plasma membranes of adjacent surface mucous cells, but more abundantly in the SMGL. This organism was not found on the metaplastic cells showing intestinal properties, regenerated immature cells surrounding ulcerated lesions and neoplastic epithelial cells of carcinoma. In the SMGL, it tended to distribute in the layers of the surface mucous cell-type mucin, and, when it existed, marked disintegration of mucous layers and vacuolation was evident. These data suggest that disintegration of mucous in the SMGL is one of the factors which accelerate mucosal damage by this agent.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 28 (2), 181-185, 1995

  日本組織細胞化学会

キーワード

• Histochemistry
• <I>Helicobacter pylori</I>
• Surface mucous gel layer
• Mucin
• Stomach

詳細情報

• CRID: 1390282679837986304
• NII論文ID: 10008976474
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.28.181
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/28/2/28_2_181/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996082265
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可