Drug Resistance of Enterohemorrhagic <I>Escherichia coli</I> O157 and a Possible Relation of Plasmids to the Drug-resistance

• MIWA Yoshio

  Aichi Prefecture, Ichinomiya Health Center

• MATSUMOTO Masakado

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• HIRAMATSU Reiji

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• YAMAZAKI Mitsugu

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• SAITO Hiroshi

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• SAITO Makoto

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• SUZUKI Yasumoto

  Aichi Prefectural Institute of Public Health, Department of Microbiology

• MIYAZAKI Yutaka

  Aichi Prefectural Institute of Public Health

Bibliographic Information

Other Title

• 腸管出血性大腸菌O157の薬剤感受性及び薬剤耐性とプラスミドの関連について
• チョウカン シュッケツセイ ダイチョウキン O157 ノ ヤクザイ カンジュセイ オヨビ ヤクザイ タイセイ ト プラスミド ノ カンレン ニ ツイテ
• Drug Resistance of Enterohemorrhagic Escherichia coli O157 and a Possible Relation of Plasmids to the Drug-resistance

Abstract

Antimicrobial susceptibility was examined using 89 enterohemorrhagic Escherichia coli O157 isolates obtained from diarrhea patients in Aichi Prefecture, Japan between June 1996 and June 1997. Among the 89 isolates, 15 (16.9%) were found to be resistant to 6 of 9 antibiotics examined. These 6 antibiotics were ampicillin (ABPC), cefaloridine (CER), chloramphenicol (CP), kanamycin (KM), streptomycin (SM), and tetracycline (TC). Among the 15 drug-resistant isolates, 7 were resistant to 4 drugs (ABPC, CER, SM, TC), 3 were resistant to 3 (ABPC and 2 of CER, SM, TC), 2 were resistant to 2 (SM, TC), one each to KM or SM. Another isolate showed resistance to 5 drugs (ABPC, CP, KM, SM, TC). Selected 13 drug-sensitive and selected 12 multi-drug resistant isolates were tested for the presence of plasmids. All of the drug-sensitive isolates had 54 MDa plasmid and the majority (8/13) had 2.0 MDa plasmids, whereas; all of the drug-resistant isolates except one (1/12) had 54 MDa plasmid and the majority had 8.0 MDa (9/12) and 4.2 MDa (11/12) plasmids. The first transformation test revealed that plasmids of 8.0MDa (3/4) and 46 MDa (1/4) were transferred to a donor cell with ABPC resistance. 54MDa plasmid was transferred to a donor cell with both of ABPC and TC resistance. In the second transformation test, only the 8.0 MDa plasmid was confirmed to be transferred to a donor cell with ABPC resistance. Accordingly, it was indicated that the ABPC resistant gene was carried on 8.0 MDa plasmid, and it was suggested that resistant genes for ABPC and TC, and ABPC were carried on 54 MDa, and on 46 MDa plasmids, respectively.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 76 (4), 285-290, 2002

  The Japanese Association for Infectious Diseases

Keywords

• enterohemorrhagic <I>Escherichia coli</I> O157
• drug resistance
• plasmid

Details

• CRID: 1390282680027689088
• NII Article ID: 130004112799
• NII Article ID: 10008978849
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.76.285
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 6150917
• PubMed: 12030028
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6150917
• Web Site: https://search.jamas.or.jp/link/ui/2002212358
• Data Source:
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  • NDL
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed