<i>Phaseolus calcaratus</i> の異なる2品種における <i>in vitro</i> での迅速なマイクロプロパゲーション

• BANSAL Yogendra. K.

  Department of Biological Sciences, R. D. University

• PANDEY Punita

  Department of Biological Sciences, R. D. University

書誌事項

タイトル別名

• Rapid In Vitro Micropropagation of Two Different Cultivars of Phaseolus calcaratus RBL-1 and RBH-35.
• Phaseolus calcaratusの異なる2品種におけるin vitroでの迅速なマイクロプロパゲーション〔英文〕
• Phaseolus calcaratus ノ コトナル 2 ヒンシュ ニ オケ

抄録

A successful protocol for rapid proliferation of adventitious shoot from hypocotyl and leaf explants of two cultivars of Phaseolus calcaratus cv., RBL-1 and RBH-35 is described. A high frequency of multiple shoot buds was obtained directly from cut ends of the explants on MS medium supplemented with different (1-10 mgl^-1) concentrations of auxins viz. 2, 4-dichlorophenoxyacetic acid (2, 4-D), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) alone and in combination with cytokinin, 6-benzyladenine (BA). Lower concentrations of auxins (0.1 and 0.5mgl^-1) alone and in combination with different concentrations of BA were in general found to be the best for multiple shoot differentiation within 3-4 weeks of culture. Rooting of in vitro regenerated shoots was induced by NAA (0.1mgl^-1) alone or a combination of IBA and BA (0.1+0.1mgl^-1). Regeneration was much higher from hypocotyl than from leaf.

収録刊行物

• 植物組織培養

  植物組織培養 12 (1), 16-19, 1995

  日本植物細胞分子生物学会

詳細情報

• CRID: 1390001204329331200
• NII論文ID: 10009423276
• NII書誌ID: AN10050931
• DOI: 10.5511/plantbiotechnology1984.12.16
• ISSN: 18847706
• ISSN: 02895773
• NDL書誌ID: 3617153
• Web Site: http://id.ndl.go.jp/bib/3617153
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3617153
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可