ギョウジャニンニクの芽生えおよび未熟はいからのカルス誘導と植物体再生

書誌事項

タイトル別名
  • Callus Formation and Plant Regeneration through In vitro Culture of Immature Embryo and Seedling in Allium victorialis L. ssp. platyphyllum Hult.
  • ギョウジャニンニク ノ メバエ オヨビ ミジュク ハイ カラ ノ カルス ユウ

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抄録

The present study was carried out to establish an efficient propagating condition of Allium victorialis L. ssp. platyphyllum Hult. through callus culture. The callus was efficiently induced from the seedling on AZ medium containing 1 to 10μM 2, 4-D, especially in addition to BA. The immature embryo was more suitable for inducing callus than the seedling. It was found that AZ medium was most effective in enhancing callus formation from the immature embryo compared with MS or BDS medium. The rate of adventitious buddifferentiation from seedling-derived callus was higher on BDS medium than on MS medium. Some pathways were recognized in plant regeneration from seedling-derived callus. Shoot growth and root differentiation were accelerated by transferring the adventitious buds to the MS medium supplemented with 1μM NAA and 1μM kinetin.

収録刊行物

  • 植物組織培養

    植物組織培養 13 (1), 15-21, 1996

    日本植物細胞分子生物学会

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