白色腐朽菌の酵素活性と物質分解特性

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書誌事項

タイトル別名
  • Enzyme Activity and Degradation Characteristics of Environmental Chemicals by Phanerochaete chrysosporium.
  • ハクショク フキュウキン ノ コウソ カッセイ ト ブッシツ ブンカイ トクセイ

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抄録

Enzyme activity of the white rot fungus Phaneroeheate chrysosporium and the degradation activity for refractory organic compounds was studied. Experiments were conducted under ligninolytic (low-nitrogen) and non-ligninolytic (high-nitrogen) culture conditions with Basal III medium containing glucose as a carbon source and ammonium tartrate as a nitrogen source. Under both high-nitrogen and low-nitrogen conditions, lignin peroxidase (LiP) activity was sufficiently produced. The level of LiP activity reached the peak and was decreased more slowly by added matter such as veratryl alcohol (VA) and nonionic surfactant (Tween-80) than nitrogen and oxygen level. However, the formation of manganese-dependent peroxidase (MnP) activity was more affected by the nitrogen level than that of LiP activity. Degradation activities for three organochlorine compounds, one organochlorine pesticide and two PAH were observed under ligninolytic and non-ligninolytic conditions. For example, the degradation of phenanthrene and γ-BHC was observed under ligninolytic as well as non-ligninolytic conditions. This indicates that LiP and MnP are not essential for the degradation of the compounds by this fungi. It is also demonstrated that the potential of P. chysosporium for degradation of certain environmental pollutants is more affected by added matters than limited nutrient starvation conditions.

収録刊行物

  • 環境化学

    環境化学 10 (1), 1-11, 2000

    一般社団法人 日本環境化学会

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