Thermal resistance traits of the clonal silver crucian carp Carassius langsdorfii and evaluation of these traits using primary culture cells.
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- SAKAMOTO KENJI
- Department of Marine Biotechnology, Fukuyama University Research Institute of Marine Bioresources, Fukuyama University
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- KOEDPRANG WORAWUT
- Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University
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- NAKAJIMA MASAMICHI
- Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University
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- TANIGUCHI NOBUHIKO
- Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University
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Abstract
The thermal resistance traits of three clonal lines of the silver crucian carp Carassius langsdorfii (SCC-1, -2 and -3) were investigated. Individual juvenile fish reared at 20°C were exposed to thermal stress at 36.5±0.5°C and their death times during this exposure were measured. The death times of SCC-2 and SCC-3 fish were 66.6±31.2 and 144.7±49.8 min, respectively. In contrast, all SCC-1 fish survived under these conditions. Furthermore, the thermal sensitivity of primary culture cells from each clone was determined at 37, 40 or 43°C by the Trypan blue assay. Under all treatment conditions, the thermal sensitivity of the SCC-1 primary cultures was lower than those of the others. These results estimated the correlation between the in vivo and in vitro thermal resistances. Therefore, the use of primary culture cells to evaluate thermal resistance could be a useful method of selection breeding.
Journal
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- Fisheries science
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Fisheries science 68 (5), 1029-1033, 2002
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001204428113408
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- NII Article ID
- 130003742470
- 10009716600
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- NII Book ID
- AA10993718
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- ISSN
- 14442906
- 09199268
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- NDL BIB ID
- 6454896
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed