Comparison of Strength of Endogenous and Exogenous Gene Promoters in Arabidopsis Chloroplasts

DOI Web Site 参考文献58件
  • YOSHIMOTO Kohki
    Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
  • SAKAIYA Mao
    Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
  • ISONO Kyoichi
    Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka Present address: Department of Molecular Embryology, Graduate School of Medicine, Chiba University
  • KOBAYASHI Hirokazu
    Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka

この論文をさがす

抄録

We have focused on possible stronger promoters in the chloroplast: those of psbA encoding D1 protein of photosystem II reaction center, 16S rDNA in rrn operon, the bacterial fused promoter tac, and the bacteriophage T7 gene φ10 in combination with transgenic T7 RNA polymerase (RNAP). Arabidopsis plants were made transgenic in the nuclear genome with the construct of a chimeric gene for T7 RNAP fused to a chloroplast transit peptide at its N-terminus placed under the control of CaMV 35S promoter. We have transiently expressed gene for β-glucuronidase (GUS) under control of the above promoters in the Arabidopsis chloroplast followed by particle bombardment. Expression in the chloroplast but not in the nucleus was confirmed histochemically and by treatment with α-amanitin. T7 promoter was the strongest among the examined promoters in the Arabidopsis chloroplast, being applicable to higher expression of foreign genes in the chloroplast with managed expression of T7 RNAP.

収録刊行物

参考文献 (58)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ