Organization and Structure of Ferredoxin. Dependent Glutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants.

DOI Web Site 被引用文献2件 参考文献55件
  • HAYAKAWA Toshihiko
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • SAKAI Takahiro
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • ISHIYAMA Keiki
    Metabolic Function Group, Plant Science Center, RIKEN Yokohama Institute
  • HIROSE Naoya
    Metabolic Function Group, Plant Science Center, RIKEN Yokohama Institute
  • NAKAJIMA Hiroyuki
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • TAKEZAWA Masae
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • NAITO Kazutaka
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • HINO-NAKAYAMA Mutsumi
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • AKAGAWA Takumi
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • GOTO Satoshi
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University
  • YAMAYA Tomoyuki
    Laboratory of Plant Cell Biochemistry, Department of Applied Plant Science, Division of Life Science, Graduate School of Agricultural Science, Tohoku University Metabolic Function Group, Plant Science Center, RIKEN Yokohama Institute

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A 5822bp long cDNA clone encoding the full length ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) protein was isolated from roots of rice (Oryza sativa L. cv. Sasanishiki). Its sequence was identical to those of partial cDNAs for Fd-GOGAT from green leaves and shoots of rice. The predicted open reading frame (4848bp) encodes a 1616 amino acid protein with a molecular mass of 175034 Da that includes a 96-amino acid presequence. The combined nucleotide sequence of genomic clones for Fd-GOGAT isolated from rice was 20899 bp long and contained an entire structural gene, a 5672bp 5’-upstream region from the first methionine and a 779bp 3’-downstream region from the stop codon. The predicted transcribed region (15.4kb) consisted of 33 exons separated by 32 introns. The evolution among diverse GOGAT proteins, expression of Fd-GOGAT gene and intracellular localization of this protein in rice roots are also described.

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