Panose Production by a Mutant of Streptomyces hygroscopicus subsp. limoneus.

• HOQUE MOHAMMAD MAHFUZUL

  Division of Bioresources Science, The Graduate School of Natural Science and Technology, Okayama University

• OHASHI TOSHINARI

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University Wako Pure Chemical Industries Ltd.

• KIMURA YOSHINOBU

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• NAKAJIMA SHUHEI

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• NAGAMINE KOJI

  Takeda Food Products, Ltd.

• HIGASHIDE EIJI

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

抄録

One of the mutant non-producers of validamycin fromStreptomyces hygroscopicus subspl imoneus JCM 4911, designated as strain GT 32, showed the accumulation of panose in the culture broth under the conditions for validamycin production by the original strain. Panose production was not detected in the culture broth of the original strain which was known to produce the antibiotic, validamycin, or in that of the other mutant strains studied. The structure of panose, O- α -D-glucopyranosyl-(1→6)-O-α-D-glucopyranosyl-(1→4)-O-α-D-glucopyranose, for the oligosaccharide specifically produced by the strain GT-32, was identified by sugar composition analysis, ion-spray mass spectrometry, methylation analysis, and 500 MHz ¹H-NMR. The maximum production (1.59 mg/ml) of panose by the strain GT32 was found in 5 d cultured broth at 37°C on a rotary shaker at 180 rpm.

収録刊行物

• Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ

  Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ 2 (1), 7-12, 1997

  日本防菌防黴学会

キーワード

• Panose production
• Transglucosylation
• lon-spray mass spectrometry
• Validamycin biosynthesis
• <I>Streptomyces hygroscopicus</I> subsp. <I>limoneus</I>

詳細情報

• CRID: 1390001204464770944
• NII論文ID: 10010967334
• NII書誌ID: AA11169621
• DOI: 10.4265/bio.2.7
• ISSN: 18840205
• ISSN: 13424815
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可