昆虫の細胞分裂促進因子検出法

DOI 日本農学文献記事索引 Web Site 被引用文献1件 参考文献13件

書誌事項

タイトル別名
  • A method for detecting cell proliferation promoting factor in insects: assay system using mitosis and DNA synthesis in the wing disc cells of Bombyx mori.
  • method for detecting cell proliferation promoting factor in insects assay system using mitosis and DNA synthesis in the Wing disc cells of Bombyx mori
  • assay system using mitosis and DNA synthesis in the wing disc cells of <i>Bombyx mori</i>

この論文をさがす

抄録

We offer a system for detecting factors promoting cell proliferation in insects. Mitosis and DNA synthesis of Bombyx wing disc cells from the last larval instar were used as markers. The stage of wing discs, the addition of 20E and insect hemolymph were examined. Differences in the number of proliferating cells after culture in Grace (G), Grace containing 5ng/ml 20E (GE) and Grace containing 5ng/ml 20E and 10% hemolymph (GEH) were observed in the V1 wing discs, while differences were not obvious in the V2 and V3 wing discs. Mitoses were observed in discs cultured in the Grace medium containing 5 or 10ng/ml 20E. The addition of hemolymph increased the mitotic number in any 20E concentration. Mitosis resumed 18hr after the addition of 20E and hemolymph, then it peaked at 36hr. Among tested vertebrate growth factors, insulin revealed a promoting effect on wing disc mitosis and DNA synthesis after a 24 or 36hr culture. Wing discs from V1 stage in the GEH medium were suitable for detecting effective factors.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (13)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ