Pharmacological Evidence for Involvement of Phospholipase D, Protein Kinase C, and Sodium-Calcium Exchanger in α-Adrenoceptor-Mediated Negative Inotropy in Adult Mouse Ventricle

• Nishimaru Kazuhide

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences

• Tanaka Yoshio

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences

• Tanaka Hikaru

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences

• Shigenobu Koki

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences

書誌事項

タイトル別名

• Pharmacological Evidence for Involvement of Phospholipase D, Protein Kinase C, and Sodium-Calcium Exchanger in .ALPHA.-Adrenoceptor-Mediated Negative Inotropy in Adult Mouse Ventricle
• Pharmacological Evidence for Involvement of Phospholipase D Protein Kinase C and Sodium Calcium Exchanger in アルファ Adrenoceptor Mediated Negative Inotropy in Adult Mouse Ventricle

抄録

The intracellular signalling pathway for α-adrenoceptor-mediated negative inotropy was studied pharmacologically in isolated adult mouse ventricle. The negative inotropy was inhibited by GF-109203X, a nonselective protein kinase C inhibitor. Phorbol 12-myristate 13-acetate also produced sustained negative inotropy, which was inhibited by KB-R7943, a Na⁺/Ca²⁺ exchanger inhibitor. The α-adrenoceptor-mediated negative inotropy was augmented by RHC-80267, a diacylglycerol lipase inhibitor, but was inhibited either by C₂-ceramide, a phospholipase D inhibitor, and high concentration of propranolol (50 μM), which inhibits phosphatidate phosphohydrolase. The inotropy was not affected by U-73122, a phospholipase C inhibitor. Lavendustin-A, a tyrosine kinase inhibitor, also inhibited the negative inotropy. These findings suggest that α-adrenoceptor-mediated negative inotropy in adult mouse ventricle is mediated by activation of tyrosine kinase, the phospholipase D-phosphatidate phosphohydrolase pathway, and protein kinase C.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 92 (3), 196-202, 2003

  公益社団法人 日本薬理学会

キーワード

• α-adrenoceptor
• inotropy
• phospholipase D
• protein kinase C

詳細情報

• CRID: 1390001205175573120
• NII論文ID: 10011209471
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.92.196
• COI: 1:CAS:528:DC%2BD3sXlvFyqtrw%3D
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 6614235
• PubMed: 12890884
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6614235
• Web Site: https://search.jamas.or.jp/link/ui/2003313894
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可