Neuronal Excitation in the Ventral Tegmental Area Modulates the Micturition Reflex Mediated via the Dopamine D1 and D2 Receptors in Rats

• Hashimoto Kiyoshi

  Department of Urology, Kinki University, School of Medicine

• Oyama Tatsuya

  Research Laboratories, Nippon Shinyaku Co., Ltd.

• Sugiyama Takahide

  Department of Urology, Kinki University, School of Medicine

• Park Young-Chol

  Department of Urology, Kinki University, School of Medicine

• Kurita Takashi

  Department of Urology, Kinki University, School of Medicine

抄録

Involvement of the dopamine D₁ and D₂ receptors in the ventral tegmental area (VTA) in the micturition reflex was investigated using female Sprague Dawley rats under urethane anesthesia. Cystometrograms during continuous infusion of warmed saline into the bladder were recorded. When intervals of bladder contraction became constant, the excitatory amino acid <sc>DL</sc>-homocysteic acid (DLH) was microinjected into the VTA and changes in the cystometric parameters were observed. The selective D₁ antagonist SCH23390 (0.3 mg/kg) or D₂ antagonist eticlopride (0.1 mg/kg) was subcutaneously injected (s.c.) 15 min prior to the DLH treatment. A low dose of DLH (3 μg) facilitated the micturition reflex, whereas a high dose of DLH (30 μg) inhibited the micturition reflex. However, a middle dose of DLH (10 μg) did not show any effect. The facilitated micturition reflex induced by a low dose of DLH was inhibited by the selective dopamine D₂ antagonist eticlopride, but unaltered by SCH23390, a selective dopamine D₁ antagonist. In contrast, the inhibitory effect induced by a high dose of DLH on the micturition reflex was suppressed by SCH23390 but not eticlopride. These results suggested that the facilitated micturition reflex might be mediated via the dopamine D₂ receptors, while the inhibitory effects on the micturition reflex was mediated via the dopamine D₁ receptors.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 92 (2), 143-148, 2003

  公益社団法人 日本薬理学会

キーワード

• <sc><font size = -2>DL</font></sc>-homocysteic acid
• dopamine D₁/D₂ receptor
• ventral tegmental area
• micturition reflex

詳細情報

• CRID: 1390001205176572672
• NII論文ID: 10011232314
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.92.143
• COI: 1:CAS:528:DC%2BD3sXkvFClu7w%3D
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 6567428
• PubMed: 12832842
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6567428
• Web Site: https://search.jamas.or.jp/link/ui/2003286476
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可