Identification of Novel Microsporogenesis - Associated Genes Encoding Proteins with a Nuclear Localization Signal.
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- BAYARMAA Gun-Aajav
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- MOROHASHI Kengo
- Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama National University
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- TAKASE Hisabumi
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- HIRATSUKA Kazuyuki
- Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama National University
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Microsporogenesis is a highly organized indispensable event for the sexual reproduction of higher plants. The process includes a series of organ development, cell differentiation and meiosis. We examined the functional genes involved in microsporogenesis by random sequencing using a cDNA library from lily zygotene stage microsporocytes and isolated two cDNAs encoding novel gene products. Deduced amino acid sequences of both gene products, designated M355 and M404 contained a cluster of basic amino acid residues that may constitute a nuclear loclization signal. RT-PCR analysis indicated that the temporal and spatial expression of M355 and M404 is associated with early stages of microsporogenesis or meiosis. Transient expression of GFP fusion proteins in onion epidermal cells revealed nuclear localization activity of both proteins. These results suggest that M355 and M404 are involved in a nuclear event during the progression of microsporogenesis in lily.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 20 (2), 137-143, 2003
日本植物バイオテクノロジー学会
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詳細情報
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- CRID
- 1390282679305824000
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- NII論文ID
- 10011250113
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD3sXksFSqsL8%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 6545545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可