Measurement of Oxidative Stress in the Rodent Brain Using Computerized Electron Spin Resonance Tomography
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- LEE Masaichi-Chang-il
- Department of Pharmacology and ESR Laboratories, Kanagawa Dental College
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- SHOJI Hirofumi
- Department of Pharmacology and ESR Laboratories, Kanagawa Dental College
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- MIYAZAKI Hiroyuki
- Department of Pharmacology and ESR Laboratories, Kanagawa Dental College
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- YOSHINO Fumihiko
- Department of Pharmacology and ESR Laboratories, Kanagawa Dental College
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- HORI Norio
- Department of Prothetics, Kanagawa Dental College
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- MIYAKE Shinjiro
- Department of Orthodontics, Kanagawa Dental College
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- IKEDA Yukio
- Department of Neurosurgery, Showa University School of Medicine
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- ANZAI Kazunori
- National Institute of Radiological Sciences
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- OZAWA Toshihiko
- National Institute of Radiological Sciences
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抄録
The strategy of this study was to improve the electronic spin resonance (ESR) application used to detect free radical-induced oxidative stress in animal models. We have developed an in vivo ESR imaging system with high-quality ESR-computed tomography (CT) images by using a nitroxyl spin probe—BBB-permeable, 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-yloxy (MC-PROXYL)—in living small animals. We first measured the distribution of MC-PROXYL in the head region of a living mouse by using ESR-CT imaging after treatment with MC-PROXYL. In the ESR-CT experiments, it was clearly observed that MC-PROXYL was well distributed in the brain of head region of a living mouse. The ESR-CT images taken after treatment with MC-PROXYL demonstrate that the decay of MC-PROXYL in the isolated brain of a spontaneously hypertensive rat (SHR) was more rapid than that in a Wister Kyoto rat. ESR-CT analysis revealed that the region of rapid decay might be located in the cerebral cortex of the isolated brain of an SHR. These results suggest that the present study of ESR-CT imaging would be a useful tool for monitoring and detecting the locations of oxidative stress in the brains of rodent animal models.<br>
収録刊行物
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- Magnetic Resonance in Medical Sciences
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Magnetic Resonance in Medical Sciences 2 (2), 79-84, 2003
日本磁気共鳴医学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196727040
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- NII論文ID
- 10011434069
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- NII書誌ID
- AA11648770
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- COI
- 1:STN:280:DC%2BD2MrjsFGrtg%3D%3D
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- ISSN
- 18802206
- 13473182
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可