Evaluation of AnaeroPack(AnP) Type as Tools of Plasmodium falciparum cultivation and Drug Sensitivity Tests.

• HARUKI KOSUKE

  Department of Infectious Diseases, Kyorin University School of Medicine

• KOBAYASHI FUMIE

  Department of Infectious Diseases, Kyorin University School of Medicine

• FUJINO TAKASHI

  Department of Infectious Diseases, Kyorin University School of Medicine

• MATSUI TOSHIHIRO

  Department of Infectious Diseases, Kyorin University School of Medicine

• TSUJI MORIYASU

  Department of Infectious Diseases, Kyorin University School of Medicine

抄録

The AnaeroPack (AnP) system (Mitsui Gas Co., Japan) was produced for the purpose of culturing anaerobic bacteria. The idea using AnP Campylo® for cultivation of Plasmodium falciparum and its successful results were firstly reported by Haruki et al. (1997). In the present study, characterisation of 3 products of AnPs, i.e., AnP CO₂® (at a gas phase of 5% CO₂ and 15% O₂), AnP Campylo® (10% CO₂ and 10% O₂) and AnP Plas® (5% CO₂ and 5% O₂), in an AnaeroPack Box Jar® (22×16×10.5 cm) was studied comparing with conditions in a gas incubator or gas chamber (4% CO₂ and 3% O₂) and in a candle jar (5% CO₂ and 15% O₂). The growth curves of malaria parasites showed no difference among these four conditions by incubation for 48 hr except for AnP Plas. The observed IC₅₀ levels of chloroquine were varied much by the difference of AnPs, gas phases, the pressure in a Box Jar and free space inside. For stabilisation of IC₅₀ testing, free space in a Box Jar should be kept equal and minimal by placing the maximal 5 test plates by adding dummy plates. By the stable results of IC₅₀, gas phases can be divided into two groups. The one involves the gas incubator, AnP Plas® and AnP Campylo®, and the other does the candle jar and AnP CO₂®. Results of chloroquine sensitivity test in the candle jar group showed more drug sensitive than in the gas incubator group. These two groups should be used properly by the purpose of field experiments. The AnP® system is proven to be a practical tool for malaria culture in the field for occupying only a small space in an incubator.

収録刊行物

• Ｊａｐａｎｅｓｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｒｏｐｉｃａｌ　Ｍｅｄｉｃｉｎｅ　ａｎｄ　Ｈｙｇｉｅｎｅ

  Ｊａｐａｎｅｓｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｒｏｐｉｃａｌ　Ｍｅｄｉｃｉｎｅ　ａｎｄ　Ｈｙｇｉｅｎｅ 29 (4), 365-370, 2001

  日本熱帯医学会

キーワード

• Malaria
• Plasmodium falciparum
• in vitro culture
• AnaeroPack®
• Microanaerophilic condition
• Chloroquine sensitivity

詳細情報

• CRID: 1390282679928663552
• NII論文ID: 130003795825
• NII論文ID: 10011518863
• NII書誌ID: AN00196023
• DOI: 10.2149/tmh1973.29.365
• ISSN: 21861781
• ISSN: 21861811
• ISSN: 03042146
• ISSN: http://id.crossref.org/issn/00225304
• NDL書誌ID: 6098024
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6098024
• Web Site: https://www.jstage.jst.go.jp/article/tmh1973/29/4/29_4_365/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002166161
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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