Development of a Conditionally Immortalized Testicular Sertoli Cell Line TRS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene

• Tabuchi Yoshiaki

  Life Science Research Center, Toyama Medical and Pharmaceutical University

• Takahashi Ri-ichi

  YS New Technology Institute Inc.

• Ueda Masatsugu

  YS New Technology Institute Inc.

• Obinata Masuo

  Institute of Development, Aging and Cancer, Tohoku University

書誌事項

タイトル別名

• Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene.

抄録

Transgenic mice and rats harboring temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene are useful for establishing cell lines from tissues. We succeeded in establishing a conditionally immortalized testicular Sertoli cell line, RT3-3, from adult transgenic rats harboring the oncogene. The cells grew at permissive (33°C) and intermediate (37°C) temperatures but not at nonpermissive temperature (39°C). Large T-antigen was expressed at 33 and 37°C, whereas the expression level was gradually decreased at 39°C, suggesting that the temperature-sensitive growth characteristics arise as a result of the function of tsSV40 large T-antigen. The cells showed biochemical features associate with normal Sertoli cells including expressions of mRNAs of sulfated glycoprotein-2 (SGP-2), transferrin (TF) and steel factor. Quantitative polymerase chain reaction revealed that nonpermissive temperature induced increase in the level of SGP-2. Moreover, levels of SGP-2 and/or TF were significantly elevated in the cells treatment with sodium butyrate and retinoic acid, inducers of cellular differentiation. To our knowledge, this is the first report of the establishment of a testicular Sertoli cell line from the transgenic rats. Thus, the conditionally immortalized cell line RTS3-3 with unique characteristics may serve as good experimental in vitro models for basic and applied biology of testicular Sertoli cells.<br>

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 28 (1), 87-95, 2003

  日本細胞生物学会

キーワード

• temperature-sensitive simian virus 40 large T-antigen
• transgenic rat
• Sertoli cell
• sulfated glycoprotein-2
• transferrin
• steel factor

詳細情報

• CRID: 1390282679673217536
• NII論文ID: 130004053834
• NII論文ID: 10011619054
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.28.87
• COI: 1:CAS:528:DC%2BD3sXjtVymsbo%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 6487235
• PubMed: 12655154
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6487235
• Web Site: http://www.jstage.jst.go.jp/article/csf/28/1/28_1_87/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003224375
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可